RESUMO
Objective To investigate the histopathological and ultrastructural changes of corneal epithelium induced by erlotinib in mice.Methods Totally 30 6-8 weeks old male BALB/c mice were divided into three groups:Control group (n =12),experimental group (n =12),another 6 mice did nothing as the blank control.Experimental group used erlotinib eye drops and control group used PBS in both eyes,four times per day.At 1 day,7 days and 14 days after the intervention,corneal fluorescence staining (FL) was observed by slit lamp and graded.On the fourteenth day after the intervention,the eye balls of mice were taken,and the histopathological and ultrastructural changes of corneal epithelium and epithelial cells were observed by optical microscope and electron microscope,respectively.And protein of cornea was measured by Western Blot.Results Before the intervention,there was no significant difference in FL scores between the experimental group and control group (P ＞ 0.05).At 1 day,7 days and 14 days,FL score of experimental group was significantly higher than the groups of non-intervention,the difference was statistically significant (all P ＜ 0.05).While FL score of control group was not statistically significant before and after intervention (all P ＞ 0.05);Compared between two groups,there were statistical differences at 7 days,14 days in FL score (all P ＜ 0.05).In the experimental group,the histopathological changes of murine corneal epithelial cells had disorderly arrangement,increased layers of cells,and the inflammatory cells.Under electron microscope,the morphology of corneal epithelial surface cells was irregular and partially detached.The number of microvilli,desmosomes and hemidesmosomes were significantly decreased when compared to the control group.The expression of p-EGFR in experimental group was significantly less than that in control group,the difference was statistically significant (P ＜ 0.05).Conclusion Erlotinib can damage the tissue structure of corneal epithelium and ultrastructure of corneal epithelial cells in mice.And the mechanism is probably that erlotinib influence the corneal epithelium by inhibiting the EGFR activation.
RESUMO
Air pollution is the over emission of harmful materials into atmosphere,causing damage to human beings and other organisms.Some researches indicate that air pollution can damage ocular surface,change the tear's ingredients,and cause some symptoms,such as red eyes,lacrimation,foreign body sensation and so on.It can also change the components of the tear and damage the epithelial cells of the eyes.The mechanisms including the direct effects of the pollutant,the secondary changes of the tear,the inflammation and the oxidative stress response.As the growing problems of air pollution,its influence on ocular surface has been attracting more and more attentions.How to prevent the damage caused by air pollution is the question that ophthalmologists should think about in the future.In this paper,we focus on the sources and ingredients of air pollution,the changes and the pathogenesis of ocular surface caused by air pollution and the summary of the studies of air pollution on the ocular surface in the past few years.Studying the influence of air pollution on ocular surface significantly contributes to the theoretical research and clinical prevention.