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Objective To study the protective effects of ?-asarone against the myocardial ischemia/ reperfusion injury (MI/RI) in cultured cardiac myocytes. Methods MI/RI model was set up with Na2S2O4, which make use of rat cardiac myocytes. The viability of cardiac myocytes (using MTT method), dehydrogenase (LDH, CK) activitices in the medium were measured. Results In MI/RI model, ?-asarone can obviously advance the viability of cardiac myocytes, and decrease the dehydrogenase (LDH, CK) activitices in the medium. Conclusion ?-asarone showed protective effects against the myocardial ischemia/reperfusion injury in cultured cardiac myocytes.
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OBJECTIVE:To study the effect of different dosage of grassleaf sweetflag rhizome on central nervous system.METHODS:The effects of different dosage of grassleaf sweetflag rhizome on the hours of sleep and hypoxia live time of mice were observed,and the effects of which on water contents of brain tissues,apoptosis,endothelin(ET),malonaldehyde(MDA),erythrocuprein(SOD),glutathione peroxidase(GSH-Px),etc.in rats with cerebral ischemia were observed as well.RESULTS:Compared with the control group,different dosage of grassleaf sweetflag rhizome could shorten the sleeping time of mice and prolong the live time of hypoxia mice;high dosage of grassleaf sweetflag rhizome could remarkably decrease the water content of brain tissue and MDA level and inhibit the apoptosis of brain tissue cells while increase the activity of SOD and GSH-Px.CONCLUSION:High dosage of grassleaf sweetflag rhizome has the strongest protective effects to central nervous system,the effects of middle and low dosage of grassleaf sweetflag rhizome are lower than that of the high dosage.
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Objective To study the effects of ?-asarone on mitochondrial membrane potential(MMP) of cardiac myocytes with ischemia reperfusion injury. Methods The cardiac myocytes model of myocardial ischemia reperfusion injury (MIRI) was induced with sodium dithionite (Na2S2O4). The primary cultured cardiac myocytes of SD neonatal rats were randomized into six groups:normal group,model control group,high-dose ?-asarone (25 ?g/mL),middle-dose ?-asarone group(12.5 ?g/mL),low-dose ?-asarone group (6.25 ?g/mL) and medium group. Mitochondrial membrane potential(MMP) of myocardial cells were measured by flow cytometry. Results Compared with the normal group,MMP in the model control group was obviously decreased(P
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Gas chromatography combined with mass spectrography (GC - MS) was used to analyze the content of Muscone, a form of natural Moschus in high purity, with N2 as the carrier gas and isopsoralen as the internal standard. Results: The linear range of Muscone was from 0.056 to 0.451 g/L, regression equation: y=0. 3454 x-0. 1076,r=0.9999,the recovery was 97.99%and its RSD was 5.0%. RSD of within - day precision was 3.85% and RSD of day - to - day was 2.20% . The contents of Muscone from three batches of natural Moschus were 1.82% (RSD= 2.50%) ,3.33%(RSD = 1.36%) and 4.40%(RSD3.85%) respectively. The method is simple, rapid, reliable, and can be used as quality control for Muscone of Moschus and its preparation.
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Object To research the percutaneous absorption of ?-asarone in various solvents in vitro. Methods To measure the concentration of ?-asarone passed through rat skin in various solvents which contained borneol and azote-ketone by HPLC, count the penetration of accumulation within 24 h and steady velocity. Results 0.1% Borneol could not promote the penetration of ?-asarone; azote-ketone could decrease the penetration of ?-asarone. The penetration of accumulation within 24 h and steady velocity of ?-asarone in 20% ethanol were (352.624?87.049) ?g/cm2, (18.902?4.840) ?g/(cm2?h), respectively. Conclusion The percutaneous absorption of ?-asarone is the best when the solvents contain no promoter.
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Objective To Study on anti-thrombosis effect of volatile oil of Acorus Tatarinowii Schott(AST) and ?-asarone.Methods Thrombosis test,blood hyperviscocity test,blood clottingtest,and in vitro fibrinolytic test were carried out to investigate the effects of volatile oil of AST and ?-asarone on the following indexes:weight of thrombus,prothrombin time(PT),activated part prothrombin time(APTT),hemorheology,clotting time,the weight of plasma fibrin clot.Results Volatile oil of AST and ?-asarone can lower the weight of venout thrombosis in rats,and prolong PT and APTT of blood plasma in rats.They can improve hemorheology in the hyperviscocity rats,especially reduce viscosity of whole blood and blood plasma obviously.They can prolong the clotting time in mice obviously;and decrease the weight of plasma fibrin clot.Conclusion Volatile oil of SCP and ?-asarone has anti-thrombosis anticoagulationand fibrinolyitc effect,and its mechanism may be related to the prolongation of PT and APTT,improving blood viscosity.
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Objective: To establish the quality control standards of two intermediate products of Xingshen Nasal Drops:musk aromatic water and volatile oil of Acorus tatarinowii Schott so that the content of main components and composition of the different batches of ultimate products remained stable.Methods: The content limit of muskone of musk aromatic water was ascertained by GC-MS. And the characteristic finger print of volatile oil of Acorus tatarinowii Schott was established. Results: The content of muskone of musk aromatic water should be controlled at the range of 0.166～0.202mg/mL. RSD of muskone content of 3 batches of ultimate products was 4.49%, not larger than 10%. The characteristic finger point of volatile oil of Acorus tatarinowii Schott was composed of 6 main component peaks: Methyleugenol, cis-Methylisoeugenol, trans-Methylisoeugenol, Elemicin, ?-Asarone and ?-Asarone. This characteristiz finger print could be repeated when the ultimate product was determined. Conclusion: The establishment of quality control standard of intermediate product can raise the product quality of compound preparations. And GC-MS is one of effective determination methods.