RESUMO
Objective To investigate the diagnostic value of IL-27, ADA and TB-Ab in TB pleurisy. Methods The data of 74 TB pleurisy cases and 45 non-TB pleurisy cases were screened randomly from June 2017 to July 2018 in Dalian Tuberculosis Hospital. Value of IL-27, ADA and TB-Ab in blood and pleural fluid of the two groups of cases was detected, and diagnostic value of these biomarkers in TB pleurisy was compared. Results The value of IL-27, ADA and TB-Ab in blood and pleural effusion of patients with TB pleurisy were higher than those of control group (P < 0.05). ROC curve analysis showed that areas under the blood IL-27, ADA and TB-Ab curves were 0.820, 0.744 and 0.589 (P<0.05) respectively, while those under the pleural effusion curves were 0.921, 0.876 and 0.708 (P<0.05) respectively. The area under the curve of IL-27 and ADA ROC curve was 0.921 (P<0.05), but 95% CI was higher than that of pleural effusion ADA (0.804-0.930) and IL-27(0.857-0.962). Conclusions Detection of IL-27 and ADA in pleural effusion is of great value in the diagnosis of TB pleurisy. The combined detection of IL-27 and ADA in pleural effusion would improve the diagnostic value.
RESUMO
The conditions of the mosquito-borne HBsAg were investigated with the RPHA method in Henan Province. The number of capture of Anopheles hyrcanus sinensis was small, and no HBsAg was detected from them. HBsAg was detected in both Culex pipiens pallens and Armigeres subalbatus and their positive rates of HBsAg were 10.3 and 4.6% respectively. The positive rates varied with different physiological conditions of mosquitoes, being 15.0, 7.2 and 7.5% respectively for fed, unfed and gravid females.With the digestion of the blood in the mosquito's stomach, most of the HBsAg was excreted in the feces gradually. This new finding of the transmission of HBsAg inside the mosquito's body has not been reported. The fact that HBsAg was not detected in eggs and larvae showed that it is not transmitted by eggs.