RESUMO
Objective To observe the effect of proanthocyanidin on the expression of nuclear factor kappa B (NF-кB) and interleukin-6 (IL-6) in rats with spinal cord injury (SCI). Methods 36 healthy adult Sprague-Dawley rats were randomly divided into proanthocyanidin treatment group (group A, n=12), methylprednisolone (MP) treatment group (group B, n=12) and model control group (group C, n=12). Al-len's method was used to establish the model of acute spinal cord injury in T9. On the 1st, 3rd and 7th days after operation, 4 rats in each group were assessed with Basso-Beattie-Bresnahan (BBB) scale and Slanting Board Test (SBT), and the expression of NF-кB and IL-6 of the spinal cord were detected with immunohistochemistry. Results The scores of BBB and SBT were better in groups A and B than in the group C 3 and 7 days after SCI (P<0.05). Compared with group C, the expression of NF-кB in group A decreased all the time after SCI (P<0.01), and 3 and 7 days after SCI in group B (P<0.01);while the expression of IL-6 decreased all the time after SCI in groups A and B (P<0.05). Conclusion Proanthocyanidin may inhibit the expression of NF-кB and IL-6 in spinal cord after SCI in order to reduce inflammation and improve the hindlimb motor function in rats after SCI.
RESUMO
Anti-lipid peroxidation actions of alcoholic extracts of common Araceae medicinal materials(Pinellia pedatisecta, Typhonium flaglliforme, Pinellia ternata) and Ranunculaceae medicinal materials (Aconitum carmichaeli,Anemone altaica)were studied. Inhibition of air-oxidation of linoleic acid by these drugs were observed in the experiments. It was found that Araceae and Ranunculaceae crude drugs showed good antioxidative effects,especially with P. pedatisecta and A. carmichaeli which showed more remarkable inhibition. Pharmacological studies showed that GSH-Px activity in the whole blood of rats fed with these two crude drugs and CAT activity in red cells of the rats were significantly higher than that in control group(P