Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 3 de 3
Filtrar
1.
Journal of Huazhong University of Science and Technology (Medical Sciences) ; (6): 839-845, 2016.
Artigo em Inglês | WPRIM | ID: wpr-238433

RESUMO

Gefitinib is used as a first-line treatment for advanced non-small cell lung cancer (NSCLC). Unfortunately, most NSCLC patients inevitably develop gefitinib resistance during treatment. In addition to EGFR mutation status, the mechanisms involved are largely unknown. In this study, we showed that miR-124, a tumor suppressor, was significantly down-regulated in gefitinib-resistant NSCLC patients and cell lines compared with gefitinib-sensitive patients and cell lines. In addition, the miR-124 depletion induced gefitinib resistance, and miR-124 overexpression sensitized gefitinib-resistant cells to gefitinib. Mechanistic analysis revealed that miR-124 decreased SNAI2 and STAT3 expression by directly targeting their 3'UTRs and that knocking down SNAI2 or STAT3 partly reversed the gefitinib resistance induced by miR-124 depletion. Our data demonstrate that the miR-124 plays a new critical role in acquired resistance to gefitinib and that the manipulation of miR-124 might provide a therapeutic strategy for reversing acquired gefitinib resistance.


Assuntos
Humanos , Regiões 3' não Traduzidas , Antineoplásicos , Farmacologia , Usos Terapêuticos , Carcinoma Pulmonar de Células não Pequenas , Tratamento Farmacológico , Genética , Metabolismo , Linhagem Celular Tumoral , Resistencia a Medicamentos Antineoplásicos , Genética , Células HEK293 , Neoplasias Pulmonares , Tratamento Farmacológico , Genética , Metabolismo , MicroRNAs , Genética , Quinazolinas , Farmacologia , Usos Terapêuticos , Fator de Transcrição STAT3 , Genética , Metabolismo , Fatores de Transcrição da Família Snail , Genética , Metabolismo
2.
Journal of Huazhong University of Science and Technology (Medical Sciences) ; (6): 224-227, 2013.
Artigo em Inglês | WPRIM | ID: wpr-343114

RESUMO

Insulin-like growth factor-I (IGF-I) is a mitogenic and anti-apoptotic factor. Serum IGF-I concentration is related to some cancer risk and tumor progression. The aim of this research was to study the association of preoperative serum IGF-I concentration with clinicopathological parameters and prognosis of non-small cell lung cancer (NSCLC). Preoperative serum IGF-I concentration was measured in 80 consecutive patients with NSCLC who underwent radical lung cancer resection, and 45 patients with benign pulmonary lesion (BPL) by using enzyme linked immunosorbent assay (ELISA). The results showed that the serum IGF-I concentration was elevated and correlated with clinicopathological parameters and overall survival (OS) in NSCLC patients. Serum IGF-I concentration was significantly higher in patients with NSCLC than in those with BPL. The IGF-I concentrations were significantly higher in NSCLC patients with ≥T2, N1-3, and in IIIA-IV but not in those with <T2, N0, or IA-IIB. The increased serum IGF-I concentration was significantly correlated with poor prognosis. Our data show the positive correlation between IGF-I serum concentration and the tumor size for the first time. It seems that IGF-I related to the progression of lung cancer may depend on autocrine/paracrine function. In addition, our study reveals that higher serum IGF-I concentration is correlated with larger tumor size, advanced stages, local lymph node metastasis and worse prognosis, indicating that endocrine IGF-I is also important for the progression for NSCLC.


Assuntos
Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Biomarcadores Tumorais , Sangue , Carcinoma Pulmonar de Células não Pequenas , Sangue , Diagnóstico , Cirurgia Geral , China , Fator de Crescimento Insulin-Like I , Neoplasias Pulmonares , Sangue , Diagnóstico , Cirurgia Geral , Período Pré-Operatório , Prognóstico , Reprodutibilidade dos Testes , Medição de Risco , Sensibilidade e Especificidade , Taxa de Sobrevida
3.
Journal of Huazhong University of Science and Technology (Medical Sciences) ; (6): 224-7, 2013.
Artigo em Inglês | WPRIM | ID: wpr-636443

RESUMO

Insulin-like growth factor-I (IGF-I) is a mitogenic and anti-apoptotic factor. Serum IGF-I concentration is related to some cancer risk and tumor progression. The aim of this research was to study the association of preoperative serum IGF-I concentration with clinicopathological parameters and prognosis of non-small cell lung cancer (NSCLC). Preoperative serum IGF-I concentration was measured in 80 consecutive patients with NSCLC who underwent radical lung cancer resection, and 45 patients with benign pulmonary lesion (BPL) by using enzyme linked immunosorbent assay (ELISA). The results showed that the serum IGF-I concentration was elevated and correlated with clinicopathological parameters and overall survival (OS) in NSCLC patients. Serum IGF-I concentration was significantly higher in patients with NSCLC than in those with BPL. The IGF-I concentrations were significantly higher in NSCLC patients with ≥T2, N1-3, and in IIIA-IV but not in those with

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA