Effect of HAART on CD4+T cell and viral load in children with AIDS combined with HBV/HCV infection / 国际检验医学杂志

Objective To investigate the effect of high performance antiretroviral therapy on CD4+T cells, viral load and liver function in children with AIDS combined with HBV/HCV infection.Methods The simple HIV from April 2014to April 2015in our hospital infection in 88cases were divided into group A, 8cases of HIV patients with HBV infection was divided into B group, and 23cases of HIV patients with HCV infection was divided into C group, three groups were all treated with HAART, after about two years treatment time, before and after treatment of liver function, viral load and CD4+T cell changes between the three groups were compared.Results Before treatment, the total of three groups of children with abnormal liver function was 29cases (24.79％) , and the three groups were not statistically significant (P>0.05) .After treatment, the total of three groups of children with abnormal liver function were 82 (70.09％) , and the difference between the three groups was statistically significant (P<0.05) .Before treatment, there was no significant difference in the levels of AIT, AST and TBIL between the three groups (P>0.05) .After treatment, the levels of AIT, AST and TBIL in the three groups increased significantly (P<0.05) .The data between the three groups were not statistically significant (P>0.05) .Before treatment, there was no significant difference between the three groups of children with CD4+T cells and viral load (P>0.05);after treatment, the three groups of patients with CD4+T (P<0.05) cells increased, HIVRNA decreased significantly (P<0.05) , the three groups had no statistically significant difference between the two groups of patients (P>0.05) .Conclusion High-efficiency antiretroviral treatment in children with AIDS complicated with HBV/HCV infection, there are different differences in liver function of patients, after treatment can significantly increase the level of CD4+T cells, reduce viral load, AIDS combined with HBV/HCV infected children have different differences, the liver function should be paid close attention in clinical application.

Changes of cerebra flow under monitoring of transcranial Dopple in posterior retroperitoneoscopic surgery / 医学研究生学报

Objective:To explore the changes of cerebra flow in retroperitoneoscopic surgery.Methods: Twenty-two patients undergoing retroperitoneoscopic surgery were selected.We measured Vm、Delt and PI with transcranial Dopple(TCD),and measured endothelin(ET),neurol peptide Y(NPY),Calcitonin gene-related peptide(CGRP) in plasma before CO2 insufflation,after CO2 insufflation,and after CO2 deflation.Results:Vm and Delt increased significantly at 30 min after pneumoperitonenum,and reached the peak at 90 min after pneumoperitonenum.PI decreased significantly 90 min after pneumoperitonenum.The plasma concentrations of ET and NPY increased significantly 60 min after pneumoperitonenum. Conclusion:Cerebra flow changes significantly in patients undergoing retroperitoneoscopic surgery,which is correlated to the changes of plasma CO2 and vascular bioactive substances.

Studies on purification and characterization of a protease from a marine bacterium, Pseudomons sp. 7~11 / 中国海洋药物

A protease from the culture supernatant of marine bacteria Pseudomonas sp. 7~11 was discovered. One component of the protease was purified to homogeneity by ammonium sulfate precipitation, dialysis, ion exchange chromatography. The specific activity of the enzyme was raised from 19.46U?mg -1 to 13953115U?mg -1 ,which was 717.02 times that of the culture supernatant with a yield of 1.24%.The molecular mass of purified enzyme was estimated to be 3000Da about by SDS PAGE. The optimum temperature for the activity was observed to be 45℃ using casein as substrate.The optimum pH for activity the enzyme was 8.0 and it was stable between pH6～7 and below 50℃.The activity of the enzyme was inhibited by EDTA and IAA strongly. But was not inhibited by PMSF. the enzyme was inhibited Mn 2+ , Hg 2+ , Fe 2+ , Zn 2+ , Cu 2+ ,Pb 2+ and SDS. The inactivity of the enzyme with EDTA could be recovered partially by Mg 2+ .while Ca 2+ , Mg 2+ and (NH 4) 2SO 4 was activator for the activity of the enzyme. The activity of the enzyme was fairly stable in the presence of ethanol and urea, and was resistant to Tween 20.

An analytical study of the functions of two phase media of liquid and gel with the microcolumn gel Coombs'test / 中国免疫学杂志

Objective:To analyse the functions of two phase media of Liquid and Gel with the Microcolumn Gel Coombs'Test and study the reasons for obviating the need of washing RBC in the Gel Coombs'T.Methods:Add tryplan blue solution into the wider upper portion of the microcolumn gel tube,observe the time of the tryplan blue solution diffusion into the Gel medium at the narrow bottom portion of the tube.Add tryplan blue solution into the liquid reactant in test tube,observe the time of the tryplan blue solution diffusion into the liquid medium.Results:In the Microcolumn of Gel Coombs'Test there is no visual mixing of the two phases for 6 h at lest which is much longer than the incubation time needed for antibody-antigen reaction in routine red cell serology.It takes more than 30 h for the 2 phases to mix.In the test tube of Coombs'Test the tryplan blue solution diffusion into the liquid reactant of serum,red cells and anti-globin reagents and turned to blue colour immediatedly.Conclusion:Two reasons for non-requirement of washing in Gel-Coombs'T have been found.Firstly,there are two phases of reactants in the tubes of cards,the AHG is present in the gel at the narrow bottom portion of the tube,and the RBC,the serum to be detected in the wider upper portion of the tube.Secondly,the gel serves as not only a medium for separating agglutinated RBC from the dispersed RBC,but as a medium for boosting the reactions of AHG with IgG sensitized RBCs.The crux of the two reasons is of the two phases of reactants.