Predictive Value of CHA2DS2-VASc Score for Contrast-induced Nephropathy After Elective Percutaneous Coronary Intervention in Patients With Coronary Heart Disease / 中国循环杂志

Objectives: To investigate the predictive value of CHA2DS2-VASc score for contrast induced nephropathy (CIN) after percutaneous coronary intervention in patients with coronary heart disease. Methods: A total of 356 patients undergoing elective percutaneous coronary intervention were enrolled in this study. The patients were divided into two groups according to the CHA2DS2-VASc score: CHA2DS2-VASc score ≥ 3 (n=153) and ≤ 2 (n=203). Baseline data, incidence of CIN and major adverse cardiovascular events were analyzed and compared between the two groups. The predictive effect of CHA2DS2-VASc score was analyzed with receiver operating characteristic curve (ROC) and logistic regression analysis. Results: Left ventricular ejection fraction was significantly lower, baseline serum creatinine value was significantly higher, coronary lesions were more complex, contrast agent dosage used was significantly larger and the incidence of CIN was significantly higher in patients of the CHA2DS2-VASc score ≥ 3 group than in patients of CHA2DS2-VASc score ≤ 2 group (all Pvalues<0.05). Multivariate logistic regression analysis showed that CHA2DS2-VASc score≥3 was an independent predictor of CIN (OR=2.152, 95% CI: 1.261-3.987, P=0.032). The area under the curve of ROC of CHA2DS2-VASc score ≥ 3 for predicting CIN was 0.749 (sensitivity 76.9%, specificity 73.0%). Conclusions: CHA2DS2-VASc score could predict the CIN after percutaneous coronary intervention in patients with coronary heart disease, which could help us identify the high-risk patients of CIN and take preventive measures to reduce the incidence of CIN post percutaneous coronary intervention.

Construction of Tumor Tissue Array on An Open-Access Microfluidic Chip / 分析化学

An open-access microfluidic chip which enabled automatic cell distribution and complex multi-step operations was developed.The microfluidic chip featured a key structure in which a nanoporous membrane was sandwiched by a cell culture chamber array layer and a corresponding media reservoir array layer.The microfluidic approach took advantage of the characteristics of the nanoporous membrane.On one side, this membrane permitted the flow of air but not liquid, thus acting as a flow-stop valve to enable automatic cell distribution.On the other side, it allowed diffusion-based media exchange and thus, mimicked the endothelial layer.In synergy with a liquid transferring platform, the open-access microfluidic system enabled complex multi-step operations involving medium exchange, drug treatment, and cell viability testing.By using this microfluidic protocol, a 10 × 10 tissue arrays was constructed in 90 s, followed by schedule-dependent drug testing.Morphological and immunohistochemical assays results indicated that the resultant tumor tissue was faithful to that in vivo.Drug testing assays showed that the microfluidic tissue array promised multi-step cell assays under biomimetic microenvironment, thus providing an advantageous tool for cell research.

Application of SNPstream for medical genetics research / 中华医学遗传学杂志

<p><b>OBJECTIVE</b>To introduce the principle, procedure, efficacy and application of SNPstream genotyping technology.</p><p><b>METHODS</b>Genotyping results of 152 SNPs were used to analyze the feasibility, call rate and accuracy of SNPstream technology.</p><p><b>RESULTS</b>For the 152 selected SNPs, 122 SNPs can be genotyped with SNPstream, for which 116 SNPs were successfully genotyped. Replication study showed that the repeatability of genotyping is 99%. When the allele cluster was clear, the accuracy can reach 100%. But when the allele cluster was obscure, the accuracy was only 93.8%.</p><p><b>CONCLUSION</b>SNPstream technology has the advantages of high accuracy, flexible throughput, and high cost performance, and may have a wide application for medical genetics research.</p>

The efficacy of collagen-hydroxyapatite composite membrane on bone regeneration / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To investigate the efficacy of a new collagen-hydroxyapatite (COL-HA) composite membrane on bone regeneration of SD rat cranial defects.</p><p><b>METHODS</b>Four defects were produced in the calvaria of 24 SD rats. The animals were divided into four groups: Empty defects without membrane (group 1); defects covered by COL-HA single-layer dense membranes (group 2); defects covered by COL-HA double-layer membranes (group 3); defects covered by Bio-Gide membranes (group 4). At 2, 4, 8 and 12 weeks after surgery, 6 rats were sacrificed and the following parameters were analyzed: Macroscopic observation, X-ray examination, descriptive histology, regenerate bone quantitative histology. Statistical analysis consisted of generalized linear models/factorial design analysis of variance and LSD-t test was performed.</p><p><b>RESULTS</b>Since two weeks after surgery, there were a small amount of bone regenerated in three groups except group 1. At 12 weeks after surgery, the opaque sclerous tissues filled with the defects in three groups, and residual membrane fragments still could be found. X-ray pictures showed the density of regenerate bone in group 3 and group 4 was closed to the original bone and greater than that of group 2. Quantitative analysis of regenerate bone showed that in initial stage, group 4 had more bone regeneration than the other groups (P < 0.05), and at 12 weeks after surgery the differences between group 4 and group 2/group 3 had no statistically significant.</p><p><b>CONCLUSION</b>The COL-HA composite membranes can guide bone regeneration of rat cranial defects. The efficacy of bone regeneration of COL-HA double-layer membrane is superior to COL-HA single-layer dense membrane, because its property is more propitious to the adherence and proliferation of osteoblasts.</p>

Age-specific prevalence of human papillomavirus by grade of cervical cytology in Tibetan women / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Human papillomavirus (HPV) is believed to be the most common sexually transmitted infection. However, little is known about the prevalence and distribution of HPV types in China. We aimed to assess the prevalence and the distribution of HPV types as well as risks for abnormal cervical cytology in women who reside in the Tibetan Autonomous Region of China.</p><p><b>METHODS</b>A cross-sectional study was performed involving a sample of 3036 women. An epidemiological questionnaire was applied and cervical specimens were obtained for liquid-based cytology and HPV DNA detection. Statistical analysis included chi-square and Logistic regression model.</p><p><b>RESULTS</b>In this population, 3.66% (111/3036) had atypical squamous cells of undetermined significance (ASCUS), 1.45% (44/3036) low-grade squamous intraepithelial lesions (LSIL) and 1.09% (33/3036) had high-grade squamous intraepithelial lesions (HSIL). Tibetan women (5.74%, 137/2387) exhibited lower abnormal cytology rates than non-Tibetan women (8.01%, 52/649, P = 0.03). The overall prevalence of HPV infection was 9.19% (279/3036). We failed to identify any differences in HPV prevalence by age. In the groups with normal, ASCUS, LSIL and HSIL, the overall HPV prevalences were 7.41% (211/2847), 24.32% (27/111), 56.82% (25/44) and 45.45% (15/33), respectively. HPV 16 (1.52%, 46/3036) was the most common type, and was also the most prevalent in women with ASCUS (8.11%, 9/111) and HSIL (15.15%, 5/33). The most common HPV type for Tibetan women was HPV 16 (1.42%, 34/2387), whereas for non-Tibetan individuals it was HPV 33 (2.31%, 15/649). Of the 279 HPV-infected women, 40 individuals (14.34%) presented with multiple HPV positivity. Women who had two pregnancies were more likely to have abnormal cytology smear (OR = 1.67; 95%CI: 1.07 - 2.61).</p><p><b>CONCLUSIONS</b>A low prevalence of HPV positivity was observed in women who reside in the Tibetan Autonomous Region of China. The prevalence of abnormal cervical cytology and HPV type distributions were different between Tibetan and non-Tibetan women.</p>

Prevalence of human papillomavirus infection in women in Tibet Autonomous Region of China / 中华妇产科杂志

Objective To determine the prevalence of cervical type-specific human papillomavirus (HPV)infection as well as risk factors associated in Tibet Autonomous Region of China.Methods A cluster sampling study was performed in Lasa,Rikaze and Naqu of Tibet.An epidemiological questionnaire was applied and 3036 cervical specimens were obtained for liquid-based cytology and HPV DNA detection.Statistical analysis included Wald Chi-square and stepwise logistic regression model.Results The overall HPV prevalence of involved 3036 women was 9.19%(279/3036),of which 7.05%(214/3036)of the women were infected by high-risk types (including 14 sorts of types) and 2.14%(65/3036)by low-risk types(including 6 sorts of types).There were no significant differences of HPV prevalence between age groups(P=0.936),race(P=0.718)and areas(P=0.746),respectively.Twenty-one types of HPV were detected,of which HPV16(1.52%) was the most common type,followed by HPV33(1.42%).HPV58(1.22%),HPV52(1.15%),and HPV31(1.05%).HPV type distribution was varied by age.Of the 279 HPV infected women.14.3%(40/279)exhibited multiple HPV infections.Independent risk factors for HPV infection were smoking(P=0.027),number of sex partners(P=0.198)and early age of first intercourse(P=0.237).Conclusion The overall prevalence of HPV infection in Tibet Autonomous Region is lower than that in China or abroad,in which the most common genotype is HPV16 and the independent risk factors for HPV infection included early age of first intercourse,smoking,and number of Bex partners.

Expression of CD21 in peripheral blood B lymphocytes of neonates / 中国当代儿科杂志

<p><b>OBJECTIVE</b>To study the cause of low immunologic function and insufficiency of immunoglobulin synthesis in neonates by detecting CD21 expression in B lymphocytes in different age group children.</p><p><b>METHODS</b>This study consisted of three age group children: 2-26 days (n=18), 6 months-2 years (n=12) and 3-12 years (n=17). CD21 expression in B lymphocytes was detected with flow cytometry. Serum levels of immunoglobulins were measured by immunoturbidimetry.</p><p><b>RESULTS</b>The percentage and the number of B lymphocytes expressing CD21 in the neonate group were significantly lower than in the other two age groups. The neonate group also showed lower mean fluorescence intension (MFI) of CD21. The percentage and the number of B lymphocytes expressing CD21 as well as the MFI of CD21 increased significantly with the age. The serum levels of IgA and IgM in the neonate group were noticeably lower than those in the other two age groups. The serum levels of IgA and IgM also increased significantly with the age.</p><p><b>CONCLUSIONS</b>Low CD21 expression in B lymphocytes may be related to low function of humoral immunity in neonates.</p>

Different Expression of CD_(21) on Peripheral Blood B Lymphocytes in Children in Different Age Periods / 实用儿科临床杂志

Objective To investigate the clinical significance of the expression of CD_(21) on peripheral blood B lymphocytes in different age periods.Methods All the cases was divided into newborn group,infant group and children group.The expression of CD_(21) on B lymphocytes were analyzed by the flow cytometry.Results 1.The number of CD_(21) on B lymphocytes of newborn group were obviously lo-(wer) than those in other two groups;and with growth of a child,the numer tended to grow up.2.Mean fluorescence intension(MFI),the expression of CD_(21) on B lymphocyte,also had the characteristic of increase with the age.Conclusions With the growing up of a child,the expression of CD_(21) on B lymphocyte has the tendency of up-regulation;which conforms to the feature of immune system development in childhood.Because of the higher expression of CD_(21) on B lymphocyte in certain age,it has the sensitivity to Epstein-Barr virus infection for children.

Expression of CD_ 21 on Peripheral Blood B Lymphocytes in Children with Infectious Mononucleosis / 实用儿科临床杂志

Objective To investigate the expression of CD_ 21 on peripheral blood B lymphocytes in children with infectious mononucleosis(IM).Methods The expression of CD_ 21 on B lymphocytes were analyzed with flow cytometry in IM group and two control groups.Results The ratio of B lymphocytes,the number of B lymphocytes expressing CD_ 21,and the number of CD_ 21 on B lymphocyte were significantly higher in IM group than two control groups(P