RESUMO
Objective:To explore the effect of "flash" traingingand traditional training on improving the insulin theoretical knowledge and identification ability of nurses in non-endocrinology department.Methods:We selected 443 nurses from the non-endocrine ward of our hospital by stratified sampling, and divided them into the experimental group (227 cases) and the control group (216 cases). Before the training, questionnaires on insulin knowledge were conducted. The experimental group was trained in the form of "flash", and the control group was trained with traditional training. After the training, the theoretical and operational results of the two groups were compared to analyze the error rate of common problems and training satisfaction. Chi-square test and t test were carried out by SPSS 22.0. Results:The scores of questionnaire about insulin identification knowledge and operation in the experimental group were significantly higher than those in the control group, ( P<0.01). The experimental group have significantly better control in insulin classification and insulin onset time than the control group ( P<0.01). The satisfaction of nurse was significantly higher in the experimental group than in the control group ( P<0.01). Conclusion:Non-endocrinology nurses are lack of theoretical knowledge and recognition ability of insulin. The "flash" training can improve their training satisfaction, and ensure the effectiveness and safety of insulin treatment.
RESUMO
Objective To investigate the transfer rate of alkaloids in extraction and purification process of Cortex Phellodendri Amurensis (CPA) and to ascertain the optimal purification technology.Methods We purified the alkaloids from Cortex Phellodendri Amurensis by AB-8 macroporous resin.With the contents of berberine hydrochloride and palmatine hydrochloride as the reference indexes,content determination of alcohol extract,sample solution and eluent from AB-8 macroporous resin were determined by HPLC.Results The optimal technology was as follows:using 8-fold 75 %(V/V) ethanol as extract,extracting for 3 times and lasting one hour for each time.The transfer rate of alkaloids was 83.75 %under the above condition.The transfer rate of alkaloids was about 80 %when the concentration of sample solution(pH value being 8 and centrifugation) was 0.1 g/mL,and the transfer rate of alkaloids in eluent was 75.14 %.Conclusion The optimum technology is stable and feasible,and the purification effect of alkaloids from Cortex Phellodendri Amurensis by AB-8 macroporus resin is good.