Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Adicionar filtros








Intervalo de ano
1.
Chinese Journal of Hematology ; (12): 101-104, 2008.
Artigo em Chinês | WPRIM | ID: wpr-262922

RESUMO

<p><b>OBJECTIVE</b>To analyze the cloning result of CD40 mutant from RPMI8226 cells, a multiple myeloma (MM) cell line, and study the change of the expressions of costimulatory molecules and the apoptosis of RPMI8226 cells after activated with CD40.</p><p><b>METHODS</b>CD40 gene mutant in RPMI8226 cell was detected by RT-PCR and DNA sequencing. The cell lines were cultured with sCD40L, L929/CD40L, soluble 5C11 (an anti-CD40 mAb) plate-bound 5C11 and their respective controls. Their growth curves, change of phenotypes and cell cycles were detected. The signalosome of CD40 on RPMI8226 cells were analyzed with laser scanning confocal microscope.</p><p><b>RESULTS</b>There was a single base substitution (TCA-->TTA) in the open reading frame of CD40 from RPMI8226 cells, resulting in the conversion of a amino acid (Ser124Leu). Only plate-bound antibody could inhibit RPMI8226 cell proliferation [(2.5 +/- 0.6) x 10(5) vs (7.8 +/- 1.2) x 10(5), P <0.05] and cause G1 arrested [(58.0 +/- 3.6)% vs (42.0 +/- 2.3)%, P <0.05]. muCD40 was translocated to CD40 signalosome while CD40 activated.</p><p><b>CONCLUSION</b>The mutated CD40 in RPMI8226 cell might decrease its affinity to CD40L, leading to the disorder of CD40 signal.</p>


Assuntos
Humanos , Anticorpos Monoclonais , Farmacologia , Antígenos CD40 , Genética , Alergia e Imunologia , Ligante de CD40 , Genética , Farmacologia , Ciclo Celular , Linhagem Celular Tumoral , Proliferação de Células , Análise Mutacional de DNA , Mieloma Múltiplo , Genética , Patologia , Mutação , Fenótipo , Transgenes
2.
Chinese Journal of Medical Genetics ; (6): 373-375, 2003.
Artigo em Chinês | WPRIM | ID: wpr-329456

RESUMO

<p><b>OBJECTIVE</b>To clarify the patients' pathogenic mechanism in an achondroplasia family not according with the genetic law of autosomal dominant inheritance disease at gene level.</p><p><b>METHODS</b>Genomic DNA from peripheral blood of all members in this family was used for amplification of the exon 10 of fibroblast growth factor receptor 3(FGFR3) gene by PCR; mutation was detected by DNA sequencing and identified by restriction endonuclease MaeIII.</p><p><b>RESULTS</b>A new mutation of A to T at nucleotide 1180 was found in patients but not in unaffected members.</p><p><b>CONCLUSION</b>Combined with pedigree analysis, it was summarized that achondroplasia patients in this family might result from this new mutation.</p>


Assuntos
Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Acondroplasia , Genética , Patologia , Sequência de Bases , DNA , Química , Genética , Análise Mutacional de DNA , Saúde da Família , Mutação , Linhagem , Proteínas Tirosina Quinases , Receptor Tipo 3 de Fator de Crescimento de Fibroblastos , Receptores de Fatores de Crescimento de Fibroblastos , Genética
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA