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ObjectiveTo investigate the effects of visual motion-induced brain-computer interface (BCI) technology on upper limb motor function and cognitive function of patients with stroke. MethodsFrom July, 2021 to March, 2022, 50 stroke patients with upper limb hand dysfunction in Shaanxi Provincial Rehabilitation Hospital were randomly divided into control group (n = 25) and experimental group (n = 25). Both groups received conventional rehabilitation therapy, in addition, the control group received passive rehabilitation training, and the experimental group received visual motion-induced BCI rehabilitation training, for two weeks. They were assessed with Fugl-Meyer Assessment-Upper Extremities (FMA-UE), modified Barthel Index (MBI) and Montreal Cognitive Assessment (MoCA) before and after treatment. Brain participation was evaluated during the whole training process of the experimental group. ResultsBefore treatment, there was no difference in the scores of FMA-UE, MBI and MoCA between two groups (P > 0.05). Two weeks after treatment, the scores of FMA-UE, MBI and MoCA improved in both groups (t > 2.481, P < 0.001), and were better in the exprimental group than in the control group (t > 2.453, P < 0.05); the mean brain participation of the experimental group increased 21% after treatment. ConclusionVisual motion-induced BCI rehabilitation training could promote the recovery of motor function of upper limb, and cognitive function of patients with stroke.
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Objective To observe the expression of tumor stem cell markers P 75NTR,Oct-4,Sox-2,Lin28 and Nanog in the tumor sphere from esophageal squamous cells carcinoma Eca 109 and identify the esophageal squamous cell cancer stem cell marker .Methods The serum-free culture method was used for generating tumor spheres: proliferation was observed in enrichment culture tumor spheres .Small tumor spheres were obtained after 5 days culture and big and round tumor spheres appeared after 14 days culture which were collected for experiments and passaged .The expression and location of P75NTR,Oct-4,Sox-2,Lin28, and Nanog were detected by immunofluorescence cytochemistry .Results The expressions of P75NTR,Oct-4 and Lin28 were positive in the center of tumor spheres and some on cytoplasm and other in nuclei of Eca109 monolayer cells.However, Oct-4 fluorescence intensity was weaker than P75NTR.The expressions of Sox-2 and Nanog were positive in cytoplasm of tumor spheres and Eca 109 monolayer cells .Conclusion The cells expressing P75NTR, Oct-4, and Lin28 in the center of the tumor sphere may be esophageal cancer stem cells .
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Objective To validate the discriminatory capacity of the new ankylosing spondylitis disease activity scores (ASDAS) in Chinese ankylosing spondylitis (AS) patients, and assess its clinical value. Method One hundred and twenty-nine patients with AS was included in the study, in which 87 were par-ticipat clinical trials with Etanercept (n=87) and 42 were participants of clinical trails with. The disease activity and treatment effecticacy were assessed by ASDAS, BASDAI and patient global assessment. Discriminatory ability of all the measures was analyzed as standardized mean difference (SMD) and (-score. Pearson's correlation, two indepen -dent samples t test and simple linear regression model were used for statistical analysis. Result The four ASDAS scores correlated well with patient global assessment (r=0.56～0.74), ESR (r=0.50～0.80) and CRP (r=0.50～0.69) both at baseline and the changes form baseline to 6 weeks after treatment. The four ASDAS outperformed BASDAI, patient global assessment, ESR and CRP in differentiating patients with different levels of disease activity and patients with different levels of change. There was little difference in performance between the four ASDAS versions. Conclusion The four ASDAS are highly discriminatory in evaluating the disease activity and the efficacy of drugs in Chinese AS patients, showing a significant value in clinical practice.
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Objective To consecutively understand the national clinical testing status and to reassure the quality-control of autoantibody detection. Methods Letter or telephone notification were conducted to the participating hospitals or departments. Autoantibodies for quality control survey included anti-nuclear anti-body (ANA), anti-double-stranded DNA (anti-dsDNA), anti-extractable nuclear antigens (A-ENA), anti-mitochondria antibody (AMA)/anti-smooth muscle antibody (ASMA), and anti-citrulline antibody (anti-CCP). Each had 3 control samples, and altogether 15 samples for testing. Sample distribution and data analysis were double-blinded. Results One hundred and two hospitals/departments participated in the national quality-control survey. The accurate rate for this survey was 70%, 88%, 93%, 85%, 79% respectively for ANA, anti-dsDNA, AMA, ASMA and anti-CCP. Anti-ENAs were further divided into anti-RNP, Sm, SSA, SSB and Scl-70 subgroups, and the accurate rate was 88%, 77%, 92%, 93% and 87% respectively. Conclu-sion Compared to the previous 4 national surveys, the accurate rates of ANA, anti-dsDNA, anti-ENAs, AMA in our country's autoantibody testing is improved, and the detection rate of ASMA and anti-CCP antibody is also increased. More hospitals and testing items can test these autoantibodies, which implies that autoantibody testing status has been improving in our country.
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The aim of the study is to evaluate the feasibility and safety of Bispectral index (BIS) monitoring in pediatric radio frequency catheter ablation. One hundred and six children aged 0. 6-12 years, scheduled for radio frequency catheter ablation, were randomly divided into two groups. In group A patients received BIS monitoring during the operation (n = 50), and the group B received modified Observer's Assessment of Alertness/Sedation (OAA/S) scaling (n = 56). The anesthesia was maintained with propofol target-controlled infusion. The intraoperative propefol target concentration was adjusted to maintain the BIS values between 55-65 in group A and OAA/S scale about 1 in group B respectively, The heart rate (HR), mean arterial pressure (MAP) and pulse oximetric saturation (SpO2) were measured before anesthetic induction, 1 min after induction, catheter puncturing and the end of operation respectively. The requirements of propofol, the times of supporting ventilation and recovery, the respiratory depression, nausea and vomiting postoperatively were also recorded. The intraoperative HR, MAP and SpO2 showed no differences between two groups, but the requirements of pmpofol, the times of supporting ventilation and recovery were less in group A than that of group B (P<0.05). All children didn't have nausea, vomiting and respiratory depression. The results suggest that in pediatric radio frequency catheter ablation, BIS monitoring has the advantages of timely adjustment of anesthetic depth, reducing anesthetic requirements, shortening the time of recovery, so as the perioperative safety can be improved.
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Objective To study the effect of Iguratimod on the collagen secretion of 3T6 cells. Method The median toxic concen- tration of Iguratimod was determination by MTT Assay. Iguratimod' s median toxic concentration to 3T6 cell was about 1142.6mg/L. Taking into the consideration of the cells performance after administration under spectacles, we chosen the administration concentration as 334.8 mg/L(DMS0:1?l/256?l) ,167.4mg/L,83.7mg/L and 41.85 mg/L. After administration of 12 hours, 24 hours, 36 hours and 48 hours, the supernatant was collected for assayed respectively. The collagens content was detected by the method of chloramine T. Results The collagen concentration of the drug concentration 334.375mg/L group increased (P
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Objective To determine the effects of acute normovolemic hemodilution combined with deliberate hypotension induced by sodium nitroprusside on glomerular filtration rate. Methods Forty rabbits weighing 2.6-4.0kg were anesthetized with intramuscular ketamine(1mg?kg-1). The animals were tracheotomized and intubated.Anesthesia was maintained with 0.5% halothane inhalation and fentanyl infusion (2?g?kg-1?min-1 ) . Carotid artery was cannulated for blood pressure monitoring and blood gas analysis and external jugular vein was cannulated for CVP monitoring and blood sampling. The animals were randomly allocated to one of four groups of ten animals in each group: control group; deliberate hypotension group;hemodilution group and combined hemodilution-deliberate hypotension group. Deliberate hypotension was induced with 0.02% sodium nitroprusside and MAP was reduced to 35-40mm Hg. In hemodilution group and combined group blood was removed from artery until Hct was reduced to 20 % -25 % and normovolemia was maintained by simultaneous infusion of Ringer's lactate solution, 3 times the volume of blood removed. Glomerular filtration rate was assessed by inulin clearance rate. 1% inulin 3 ml was injected I. V. over 30 s and blood samples were taken from CVP line for determination of blood inulin concentration at 1,3,5, 10, 30, 60, and 100 min after injection of inulin. Results Urine output decreased significantly in hypotension group and increased in hemodilution group. There was no significantly difference in urine output between control and combined groups. Inulin clearance decreased significantly in hypotension group as compared with that in control group, but there was no significantly difference in inulin clearance between control and combined groups. Conclusions Deliberate hypotension can decrease renal blood flow and deliberate hypotension combined with hemodilution can maintain kidney microcirculation.
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Objective To observe the effects of Baizhu Huangqi Decoction and its effective-part prescription on mice ulcerative colitis(UC).Methods The mice UC model was induced by clyster with 2,4,6-trinitrobenzenesulfonic acid(TNBS)and the effects were comprehensively evaluated by disease activity index,the macroscopic and histological assessment of colon mucosa damage and the activity of myeloperoxidase(MPO).Results Both Baizhu Huangqi Decoction and its effective-part prescription had effects on disease activity index(DAI),inflammation index and MPO activity.The effect of effective-part prescription was better than that of UC model group(P