Mechanism of Chaishao Liujunzi Tang in Treatment of Ulcerative Colitis Through TLR4/MyD88/NF-κB Signaling Pathway / 中国实验方剂学杂志

ObjectiveTo investigate the preventive and curative effect of Chaishao Liujunzi Tang （CSLJZT） on colonic mucosal injury induced by dextran sulfate sodium （DSS） in mice with ulcerative colitis （UC） and its mechanism. MethodFifty Balb/c male mice were randomly divided into normal group， model group， CSLJZT low-dose group， CSLJZT high-dose group， and sulfasalazine group. Except for the normal group， other groups were given 2.5% DSS freely for 7 d， and were given drug intervention after successful modeling for 7 d. Bodyweight， feces， and other general physiological statuses of mice were recorded every day， and disease activity index （DAI） scores were calculated.The colon length was measured， and stained by hematoxylin-eosin （HE） staining to observe the morphological changes of the colon.The enzyme-linked immunosorbent assay （ELISA） was used to determine the content of interleukin-1β （IL-1β）， myeloperoxidase （MPO）， and superoxide dismutase （SOD） in the serum. Western blot was used to determine the protein expression levels of Toll-like receptor 4 （TLR4）， myeloid differentiation factor 88 （MyD88）， nuclear factor kappa B （NF-κB）， inhibitor-kappa binding protein （IκB）， Caspase-1， and nucleotide-binding oligomerization domain-like receptor 3 （NLRP3） in the colon tissues. ResultAs compared with the normal group， mice in the model group had significantly decreased body weight （P<0.01）， severe diarrhea and hematochezia， and significantly increased DAI score （P<0.01）. As compared with the model group， the decreasing trend of body weight was significantly alleviated in the CSLJZT groups （P<0.01）， diarrhea and hematochezia were significantly improved， DAI score was significantly decreased （P<0.01）， and colon length increased （P<0.05）. HE staining showed that the pathological damage of colon tissue was significantly improved and the inflammatory cell infiltration was reduced in the CSLJZT groups as compared with the model group. As compared with the normal group， the serum levels of IL-1β and MPO were significantly higher （P<0.01） and SOD levels were significantly lower （P<0.01） of mice in the model group.Compared with the model group， the treated group reduced the serum IL-1β and MPO levels （P<0.01）， and raised the SOD level （P<0.01）. The results of Western blot showed that as compared with the normal group， the expression levels of TLR4， MyD88， NF-κB， Ccaspase-1， and NLRP3 proteins were significantly increased （P<0.01）， whereas the expression level of IκB protein was significantly decreased （P<0.01） in the colonic tissue of mice in the model group. As compared with the model group， the expression levels of TLR4， MyD88， NF-κB， Caspase-1， and NLRP3 proteins were decreased （P<0.01）， whereas the expression level of IκB protein was increased （P<0.01） in the colonic tissue of mice in the CSLJZT groups. ConclusionCSLJZT improves the inflammatory injury of the colon tissue in DSS-induced UC mice through TLR4/MyD88/NF-κB signaling pathway.

Mechanism of Chaishao Liujunzi Tang in Treatment of Ulcerative Colitis Through TLR4/MyD88/NF-κB Signaling Pathway / 中国实验方剂学杂志

ObjectiveTo investigate the preventive and curative effect of Chaishao Liujunzi Tang （CSLJZT） on colonic mucosal injury induced by dextran sulfate sodium （DSS） in mice with ulcerative colitis （UC） and its mechanism. MethodFifty Balb/c male mice were randomly divided into normal group， model group， CSLJZT low-dose group， CSLJZT high-dose group， and sulfasalazine group. Except for the normal group， other groups were given 2.5% DSS freely for 7 d， and were given drug intervention after successful modeling for 7 d. Bodyweight， feces， and other general physiological statuses of mice were recorded every day， and disease activity index （DAI） scores were calculated.The colon length was measured， and stained by hematoxylin-eosin （HE） staining to observe the morphological changes of the colon.The enzyme-linked immunosorbent assay （ELISA） was used to determine the content of interleukin-1β （IL-1β）， myeloperoxidase （MPO）， and superoxide dismutase （SOD） in the serum. Western blot was used to determine the protein expression levels of Toll-like receptor 4 （TLR4）， myeloid differentiation factor 88 （MyD88）， nuclear factor kappa B （NF-κB）， inhibitor-kappa binding protein （IκB）， Caspase-1， and nucleotide-binding oligomerization domain-like receptor 3 （NLRP3） in the colon tissues. ResultAs compared with the normal group， mice in the model group had significantly decreased body weight （P<0.01）， severe diarrhea and hematochezia， and significantly increased DAI score （P<0.01）. As compared with the model group， the decreasing trend of body weight was significantly alleviated in the CSLJZT groups （P<0.01）， diarrhea and hematochezia were significantly improved， DAI score was significantly decreased （P<0.01）， and colon length increased （P<0.05）. HE staining showed that the pathological damage of colon tissue was significantly improved and the inflammatory cell infiltration was reduced in the CSLJZT groups as compared with the model group. As compared with the normal group， the serum levels of IL-1β and MPO were significantly higher （P<0.01） and SOD levels were significantly lower （P<0.01） of mice in the model group.Compared with the model group， the treated group reduced the serum IL-1β and MPO levels （P<0.01）， and raised the SOD level （P<0.01）. The results of Western blot showed that as compared with the normal group， the expression levels of TLR4， MyD88， NF-κB， Ccaspase-1， and NLRP3 proteins were significantly increased （P<0.01）， whereas the expression level of IκB protein was significantly decreased （P<0.01） in the colonic tissue of mice in the model group. As compared with the model group， the expression levels of TLR4， MyD88， NF-κB， Caspase-1， and NLRP3 proteins were decreased （P<0.01）， whereas the expression level of IκB protein was increased （P<0.01） in the colonic tissue of mice in the CSLJZT groups. ConclusionCSLJZT improves the inflammatory injury of the colon tissue in DSS-induced UC mice through TLR4/MyD88/NF-κB signaling pathway.

Invasive mucinous adenocarcinoma with lepidic-predominant pattern coexisted with tuberculosis: a case report / 医学前沿

We observed a rare case of invasive mucinous adenocarcinoma (IMA) with a lepidic-predominant pattern accompanied by pulmonary tuberculosis. An 85-year-old man with repeated cough and sputum was admitted to Xinhua Hospital. T-SPOT test result was 212 pg/ml (reference value of negative is < 14 pg/ml), Mycobacterium tuberculosis culture was positive, and tuberculin skin test (PPD) was negative (skin induration < 5 mm). The patient was treated with several courses of antibiotics and anti-tuberculosis treatments. Repeated chest CT scans showed disease progression. Bronchoscopy yielded negative results. PET-CT scans showed negative results. A percutaneous lung biopsy revealed mucin-secreting cells lining the alveolar walls. IMA with a lepidic-predominant pattern was diagnosed after invasiveness was found after experimental treatments. Simultaneous occurrence of pulmonary tuberculosis and lung cancer are common; however, the present case of IMA having a lepidic-predominant pattern and coexisting with active tuberculosis has not been reported yet.

The effect of aluminum trichloride on expression of phosphorylated tau and Aβ in SH-SY5Y cells / 中华劳动卫生职业病杂志

Objective@#To investigate the expression of phosphorylated tau (p-tau) and Aβ in SH-SY5Y cells induced by aluminum or/and ApoE ε4 allele, and study the interaction between aluminum and ApoE ε4 allele.@*Methods@#SH-SY5Y cells were assigned to control group, 400 μmol/L AlCl3 group, ApoEε4 transfected group and 400 μmol/L AlCl3 with Apo Eε4 transfected group. The cell viability was measured by CCK-8 assay; the expressions of p-tau and Aβ was determined with ELISA Kit after AlCl3 exposure or or/and ApoE ε4 transfection.@*Results@#The viability of cells exposed to 400 μmol/L AlCl3 or/and ApoE ε4 transfected were significantly lower than that of controls (P<0.05) . The expressions of total tau, tau-181, tau-231, tau-396 and Aβ in 400 μmol/L Al3+ or/and ApoE ε4 transfected exposed cells showed significantly higher than those of controls (P<0.05) . Based on the factorial design, a significant interaction exists, and there is a synergistic effect between AlCl3 and ApoE ε4 (P<0.05) .@*Conclusion@#Aluminum and ApoE ε4 allele could increase expression of p-tau and Aβ deposition; there was a synergistic interaction between aluminum and ApoE ε4 allele on cell death, tau phosphorylation and Aβ deposition of SH-SY5Y.

Role of oxidative stress in endoplasmic reticulum stress? induced apoptosis of alveolar macrophages triggered by quartz dust / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To investigate the role of oxidative stress in the endoplasmic reticulum stress-induced apoptosis of alveolar macrophages triggered by quartz dust.</p><p><b>METHODS</b>Seventy-two healthy adult Wistar rats were randomly divided into control group, quartz dust group, quartz dust plus N-acetyl cysteine (NAC) group, and NAC group, with 18 rats in each group. One milliliter of sterile saline (for the control and NAC groups) or 1 ml of saline with 5%ultrafine quartz dust (for dust group and dust plus NAC group) was given to each rat by non-exposed endotracheal infusion. From the second day after dust infusion, rats in dust plus NAC group and NAC group received intragastric administration of NAC (100 mg/kg). In each week, the treatment with NAC lasted for 5 consecutive days, followed by 2 days' interval. For each group, 6 rats were randomly selected on the 14th, 28th, or 56th day after dust exposure; they were sacrificed by bloodletting from the femoral artery, and the lungs were collected. Bronchoalveolar lavage fluid was collected to separate macrophages. The protein expression of caspase-12 in alveolar macrophages, the apoptosis rate and reactive oxygen species (ROS) content of alveolar macrophages, and the protein carbonyl content of alveolar macrophages were determined by Western blot, flow cytometry, and colorimetry, respectively.</p><p><b>RESULTS</b>Increased protein expression of caspase-12, apoptosis rate, and content of ROS and protein carbonyl were discovered on the 14th day in the dust group, in comparison with the control group (P < 0.05), and the increase lasted till the 28th and 56th days. (P < 0.05). Compared with the dust group, the dust plus NAC group showed significant decreases in the content of ROS on the 14th, 28th, and 56th days (P < 0.05), significant decreases in the content of protein carbonyl on the 28th and 56th days (P < 0.05), and significant decreases in the protein expression of caspase-12 and apoptosis rate (P < 0.05).</p><p><b>CONCLUSION</b>Oxidative stress is potentially involved in the endoplasmic reticulum stress-induced apoptosis of alveolar macrophages triggered by quartz dust. Oxidative damage of protein in the endoplasmic reticulum may play an important role in the process.</p>

Anti-tumor peptide of tumstatin in the treatment of the transplantable model of human laryngeal squamous carcinoma in nude mice / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To study the effect of anti-tumor peptide of tumstatin on tumor growth of human laryngeal squamous carcinoma in nude mice and the underlying mechanism.@*METHOD@#Nude mice model bearing laryngocarcinoma were established by using human laryngeal squamous carcinoma cell line (Hep-II). The animals were given tumstatin or PBS for 10 consecutive days. The volumes of the subcutaneous tumor were observed. The microstructure in which the general 2-step immunohistochemical examination was adopted and ultra-micro-structural changes of carcinoma after administration of tumstatin were observed under light and electron microscopes for pathology examination.@*RESULT@#The differences was statistically significant in the net mice weight, tumor weight, tumor volume and tumor weight/net mice weight between the treatment group and the control group (P<0.01). The restrained percentage of tumor was 51.58%. The necrosis and apoptosis of the tumor cells and the angiogenesis reduction were found under light and electron microscope in the treatment group. MVD of the treatment group was lower than that of the control group (P<0.01).@*CONCLUSION@#Tumstatin can significantly restrain the development of laryngocarcinoma.

Case-control study on risk factors of laryngeal cancer in Heilongjiang province / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To study the incentives of laryngeal cancer in Heilongjiang province.@*METHOD@#A 1:One matched case control study was used to study the risk factors of laryngeal cancer in Heilongjiang province, distributing all tested staff by the same gender, age, urban and rural. Logistic regression models were used to analysis the relationship.@*RESULT@#In single Logistic regression models, such habit as high levels of education, frequently consumption of sauerkraut, BBQ food, processed meats, the less physical activity, a relatively short time, smoking, irascible, and other factors would increase the risk of suffering from laryngeal cancer. But regular consumption of fresh vegetables, coarse grains, eggs, milk, and regular physical activity would reduce the risk of suffering from laryngeal cancer. The odds ratios (OR) were calculated using multiple Logistic regression models, ORs for the highest versus the lowest quintile of intake were 15.502 0 for high levels of education. 8.012 0 for smoking frequently. 7. 2680 for eating sauerkraut. 2.904 0 for eating BBQ food. 0.408 0 for exercise in protective factors.@*CONCLUSION@#Potential risk factors for laryngeal cancer were eating sauerkraut. BBQ food and smoking frequently, but proper exercise may reduce the risk of laryngeal cancer.

Retrospective Analysis of Death Causes and Related Factors among Psychiatric Inpatients over 10 Years: 159 Cases Report / 中国康复理论与实践

@#ObjectiveTo explore the death causes and its related factors among psychotic inpatients.MethodsThe medical records of dead psychotic inpatients over recent ten years were investigated with self-designed scheme.ResultsAmong all the investigated cases, the percentage of schizophrenia was more than 50%, the death diagnosis due to somatopathy was over 80%, and more than 50% inpatients suffered from 2～3 kinds of somatopathy. Marital status, diagnosis, smoking and drinking had influenced to the live time of psychotic inpatients.ConclusionThe death of psychotic inpatients might be influenced by multiple causes, including social factors and clinical features which should be paid much attention to extend their live time.

Clinical nursing care of the degenerative lumbar scoliosis with posterior surgical treatment / 中国实用护理杂志

Objective To improve the surgical outcomes and decrease the complication rate through ac-tive and effective nursing care in patients with degenerative lumbar scoliosis (DLS) treated by posterior surgery. Methods Detailed psychological assessment, nursing care preoperafion, close observation postoperation, early prevention of the complications, active rehabilitation training were given to 34 cases of DIS patients surgically treated and effect of good clinical nursing on rehabilitation of patients was observed. Results All 34 patients with DLS had good recovery after posterior surgery, no complication occuned because of inappropriate nusing care. Conclusions Elevating nursing care levels of patients with DLS has a significant sense on improving the clinical outomes, decrease the complications and accelerate patients' rehabilitation.

The effect of atorvastatin treatment on C-reative protein and vascular cell adhesion molecule-! in the patients with acute cerebral infarction / 中国基层医药

Objective To observe the effect and difference to C-reactive protein(CRP) and vascular cell ad- hesion molecule.1 (VCAM-1), for indicator of inflammainfarction and blood lipid level in acute cerebral( ACI) pa- tients treated with atorvastatin and discuss the impact of early treatment of ACI using statin. Methods 90 ACI in- patients were randomly divided into two groups:one group treated with atorvastatin and the other was not treated with atorvastatin. Their blood levels of TG, TC, LDL-ch, HDL-ch, CRP and VCAM-1 were compared before hospi- talization and on discharge. Results Comparing before admission and on discharge, the blood level of CRP and VCAM-1 were reduced markedly in both group,the blood CRP and VCAM-1 of the former reduced significantly in atorvastatin treatment group and compared with that of the latter on discharge(P < 0.05 ). Conclusion The blood CRP and VCAM-1 level reduced markedly in ACI patients treated with atorvastatin,which suggests that atorvastatin has strong anti-inflammatory effect.

Clinical Features of First-episode Schizophrenic Patients with and without Antitoxoplasma IgG / 中国心理卫生杂志

Objective:To investigate the relationship between Toxoplasma infection and schizophrenia by comparing the clinical symptoms between the antitoxoplasma-seropositive and seronegative patients with schizophrenia.Methods: Six hundred cases of first-episode schizophrenia were examined the serum reactivity to IgG antitoxoplasma antibody by Enzyme Linked Immunosorbent Assay (ELISA). And the clinical symptoms of the schizophrenic patients were scored by use of Positive and Negative Syndrome Scale (PANSS). Results: Compared to IgG-seronegative schizophrenia, IgG-seropositive schizophrenia group was higher in the positive subscale score, the positive component score, the excitement component score, and the cognitive component score(20.8?4.2与19.4?3.4,P