The role of cell apoptosis in glioma chemotherapy evaluation / 复旦学报（医学版）

Objective To study the role of cell apoptosis during the chemotherapy of human gliocytoma in order to get effective suvilliance on the effect of chemotherapy. Methods Gliocytoma cells were isolated and cultured from 40 human gliocytoma samples. Mitochondrial membrance potential (MMP), cell cycle, the level of Bcl-2 and Bax were detected by flow cytometry (FCM) at 24, 48, 72 hours respectively of incubation with Lomustine (CCNU) and Teniposide (VM-26), and the trends were also analysed. Results MMP decreased greatly, the apoptosis part in the cell cycle ananlysis increase, the expression level of Bcl-2 decreased, and that of Bax increase rapidly, while the Bcl-2/Bax ratio decreased. Conclusions CCNU and VM-26 have singnificant effect in gliocytoma chemotherapy on inducing gliocytoma cell apoptosis. VM-26 has more stronger effect on the cell cycle. MMP is the most sensitive and the fastest index in apoptosis detection.

Combined therapy of methylprednisolone and brain-derived neurotrophic factor promotes axonal regeneration and functional recovery after spinal cord injury in rats / 中华医学杂志(英文版)

<p><b>OBJECTIVE</b>To investigate the effects of combination therapy with methylprednisolone (MP) and brain-derived neurotrophic factor (BDNF) on axonal remyelination and functional recovery after spinal cord injury in rats.</p><p><b>METHODS</b>Forty-five rats were randomly divided into three groups: Group A received MP and BDNF; group B received MP and cerebrospinal fluid (CSF); and group C received CSF only. Contusion injury to adult rat spinal cord was produced at the T(10) vertebra level followed by immediate intravenous MP or CSF, and was thereafter infused intrathecally with BDNF or CSF for 6 weeks. Axonal remyelination and functional recovery was observed using RT-PCR, immunohistochemistry and open field locomotion.</p><p><b>RESULTS</b>An increase of 28.4% +/- 2.3% in the expression of proteolipid protein (PLP) gene, an endogenous indicator of axonal remyelination, was demonstrated in group A 24 hours after injury. Ten weeks later, there were significant decreases in hematogenous inflammatory cellular infiltration in groups A and B compared to C (P < 0.05). Concomitantly, a significant amount of axonal remyelination was observed in group A compared to groups B and C (P < 0.05). Furthermore, combination therapy using MP and BDNF in group A resulted in stimulation of hindlimb activity as well as improvement in the rate of functional recovery in open field locomotion (P < 0.05).</p><p><b>CONCLUSIONS</b>Combined therapy of MP and BDNF can improve functional recovery through mechanisms that include attenuating inflammatory cellular infiltration and enhancing axonal remyelination at the injury site. Such a combination may be an effective approach for treatment of spinal cord injury.</p>

Diagnosis and treatment of intramedullary hemangioblastoma of cervical spinal cord / 中华医学杂志(英文版)

<p><b>OBJECTIVE</b>To investigate the diagnosis and surgical techniques of intramedullary hemangioblastoma of the cervical spinal cord.</p><p><b>METHODS</b>MR imaging and the methods and results of surgery were analyzed in 21 patients.</p><p><b>RESULTS</b>The tumors were divided into three types on MR imaging. Syringeal type, where the tumor varied in size and was accompanied by syringobulbia and syringomyelia; Cystic type, where the tumor presented as a cyst with a small mural node; and Solid type, where the tumor was revealed as a huge solid mass. All tumors were totally removed and diagnosis was confirmed by histological study. Post-operative neurological status was improved in 20 patients and aggravated in 1.</p><p><b>CONCLUSIONS</b>The localization and the nature diagnosis of the tumor can be made by cervical MR imaging. Operative methods vary with tumor types. It is the most important that the tumor is dissected along the right interface and removed after devascularization.</p>

Study on motor evoked potential of Arnoid Chiari malformation / 中国康复理论与实践

@# Objective To study the electrical stimulation motor evoked potentials in Arnoid Chiari malformation.Methods Electrical stimulation motor evoked potentials were recorded in 30 cases with Arnoid Chiari malformation proved by MRI.Results Abnormality rate of MEP was 96.7%,and the abnormal patterns mainly consisted of the delay of latency and the delay of central motor conduction time(CMCT). The change of MEP was connected with myodynamia and the type of Arnoid Chiari malformation .Conclusions The change of MEP is more sensitive than clinical change, and can indicate the damage of the motor function objectively and quantitatively, and MEP can be used as an objective criterion for surgical effect, and can predict the recovery of motor function.

Diagnosis and treatment of primary angitis of central nervous system / 中国神经精神疾病杂志

Objective 　To investigate methods for the diagnosis and treatment of primary angitis of central nervous system (PACNS). Methods　Radiological and clinical feature, operative resutls of 12 patients with mass lesion presentations of PACNS were analyzed retrospectively. Results　The lesions showed low density on CT and long T1 and long T2 signal on MRI. The ring-wall enhanced lesions on MRI were flowery in 7 patients. Gross total resection of the lesions were performed in 10 patients with excellent recovery postoperatively. Growth of lesions were observed in 2 patients who underwent subtotal resection. Conclusions　 Lesions of PACNS have special appearance on enhanced MRI. More patients with mass lesion presentations of PACNS could be diagnosed preoperatively according to radiological and clinical feature.For these patients, surgery is the optimal treatment at present.