Changes of telemetry electrical activity in the infralimbic cortex of morphine-dependent rats with extinguished drug-seeking behavior / 南方医科大学学报

<p><b>OBJECTIVE</b>To investigate the changes of telemetry electrical activity in the infralimbic cortex (IL) of morphine-dependent rats with extinguished drug-seeking behavior.</p><p><b>METHODS</b>SD rats were randomly divided into model group and control group and received operations of brain stereotaxic electrode embedding in the IL. The rats in the model group were induced to acquire morphine dependence and then received subsequent extinction training, and the changes of electrical activity in the IL were recorded with a physical wireless telemetry system.</p><p><b>RESULTS</b>In rats with morphine dependence, the time staying in the white box was significantly longer on days 1 and 2 after withdrawal than that before morphine injection and that of the control rats, but was obviously reduced on days 1 and 2 after extinction training to the control level. Compared with the control group, the morphine-dependent rats on day 2 following withdrawal showed significantly increased β wave and decreased δ wave when they stayed in the white box but significantly increased δ wave and decreased α wave and β wave when they shuttled from the black to the white box. On day 2 of extinction, the model rats, when staying in the white box, showed significantly decreased θ wave compared with that of the control rats group but decreased β wave and θ wave and increased δ wave compared with those in the withdrawal period. When they shuttled from black to white box, the model rats showed decreased δ wave and increased α wave and β wave compared with those in the withdrawal period.</p><p><b>CONCLUSION</b>Morphine-dependent rats have abnormal changes of electrical activity in the IL in drug-seeking extinction to affect their drug-seeking motive and inhibit the expression and maintenance of drug-seeking behaviors.</p>

Analysis of Electroencephalogram Sample Entropy Measurement in Frontal Association Cortex Based on Heroin-induced Conditioned Place Preference in Rats / 生物医学工程学杂志

To explore the relationship between the drug-seeking behavior, motivation of conditioned place preference (CPP) rats and the frontal association cortex (FrA) electroencephalogram (EEG) sample entropy, we in this paper present our studies on the FrA EEG sample entropy of control group rats and CPP group rats, respectively. We invested different behavior in four situations of the rat activities, i. e. rats were staying in black chamber of videoed boxes, those staying in white chamber of videoed boxes, those shuttling between black-white chambers and those shuttling between white-black chambers. The experimental results showed that, compared with the control group rats, the FrA EEG sample entropy of CPP rats staying in black chamber of video box and shuttling between white-black chambers had no significant difference. However, sample entropy is significantly smaller (P < 0.01) when heroin-induced group rats stayed in white chamber of video box and shuttled between black-white chambers. Consequently, the drug-seeking behavior and motivation of CPP rats correlated closely with the EEG sample entropy changes.

Telemetry electroencephalograph changes of temporal association cortex in heroin-induced conditioned place preference rats / 中南大学学报(医学版)

OBJECTIVE@#To analyze the relationship between the electroencephalograph (EEG) changes of temporal association cortex (TeA) and the drug-seeking behavior in heroin-induced conditioned place preference (CPP) rats.@*METHODS@#The rats were randomly divided into an operated control group and a heroin-induced CPP group after the electrodes were buried in TeA by stereotactic technology. The TeA EEG was recorded by the CPP video system combining with the EEG wireless telemetry, where the rats stayed in black or white chambers, shuttling from black to white chambers or from white to black chambers.@*RESULTS@#Compared with the operated control group, the percentage of TeA θ waves was increased significantly when staying in black or white chambers in the heroin-induced CPP group (P<0.05, P<0.01). Compared with the operated control group, when rats shuttling between the 2 chambers, the TeA δ waves were reduced (P<0.01), but β waves, β2 waves in particular, were increased (P<0.01) in the heroin-induced CPP group (P<0.05, P<0.01). Compared with staying in the black chamber, when heroin-induced CPP rats were shuttling between black and white chambers, the right TeA θ waves were reduced, and β waves, β2 waves in particular, were increased (P<0.01). Compared staying in the white chamber with shuttling between white and black chambers in the heroin induced CPP rats, the right TeA θ waves, but not β waves, were reduced (P<0.01).@*CONCLUSION@#The EGG changes on the right TeA in the heroin-induced CPP rats, including the increased fast waves (β, β2) and the reduced slow wave (θ), may be related to drug-seeking behaviors.

Discussion on collective preparation for classes of specialized basic subject in medical college / 中华医学教育探索杂志

Collective preparation for classes is a very effective teaching activity.It can maximize teaching effects,practically raise teacher's teaching enthusiasm for the course and improve teaching quality.Moderator of collective class preparation should be well familiar with the teaching program and content,dig into teaching material,look up for relative information,draw up standard original text then lead and organize whole preparation process for classes from the aspects of introducing teaching objective and content,analyzing teaching material,discussing the new content and progress,analyzing the relative excises,exploring the teaching methods and proposing the questions for discussion.

Thoughts and Experience of Teaching in Anesthesia-physiology To the Students in Anesthesia Medicine / 中华医学教育探索杂志

Teaching anesthesia-physiology is a subject worth to be discussed.Arranging the high quality teachers to teach,making a careful study and preparation of the teaching materials,teaching plan and enhancing the teachers’teaching art should be first emphasized.And then the advantages of using multimedia should be given full play in combination with various kinds of methods to let the students become active class participants so as to improve the teaching effect in anesthesia-physiology.

Effects of heroin at different concentrations on electrophysiological properties of rat hippocampal CA1 pyramidal neurons / 中国病理生理杂志

AIM: To investigate changes of electrophysiological properties of rat hippocampal CA1 pyramidal neurons in perfusion with heroin at different concentrations. METHODS: The intracellular recordings were made from CA1 pyramidal neurons of isolated hippocampal slices in perfusion with 0.03-0.3 mmol?L -1 heroin. Compared with control parametes before herion perfusion, the electrophysiological data obtained from identical neurons at 15 min after acute perfusion of the different concentration heroin were analyzed. RESULTS: With the increase of the heroin concentrations in perfusion, the absolute values of neuron resting potential and threshold potential decreased (P