RESUMO
Effect of environmental conditions including temperature, pH, shaking rate, ratio of medium to flask volume and inoculum percentage was studied to optimize conditions for the production of polygalacturonase by Volvariella volvaceae. No enzyme production was detected at 39C or when the fungus was grown under static conditions. The polygalacturonase enzyme was optimally produced at 34C, pH 4.5, 1% inoculum and 100 rpm shaking rate using two production media. The ratios between the medium to flask volume were 1: 3.3 and 1: 5 when the production medium contained 0.0008% CoCl2 or 0.525% MgSO4.7H2O, respectively
Assuntos
Poligalacturonase/biossínteseRESUMO
A survey was performed to analyze the tannein, ash and fiber content of 26 marketable tea samples. The data proved the presence of 4 categories of the tested tea samples with respect to tannein, i.e. 11 to 13%, 14 to 15%, 16 to 17% and from 18 to 19%. The fiber content of the investigated samples showed an average of 9.82-11.5% [DM] for one group and from 11.9-13.98% for the other group. Similar findings were noticed for the ash content; two groups of an average of 5.65% and 7.62%, respectively. Analysis of variance indicated the presence of a significant difference among the tested tea samples