RESUMO
To compare the morphology and function of corneal enthothelial cell in early postoperative period after phacoemulsification with infusion or bolus intracameral adrenaline. In this prospective randomized study, 71 eyes of 71 patients scheduled for phacoemulsification were randomly assigned to two groups: one group [31 eyes] received bolus intraocular adrenaline at 1/10,000 concentration and the other group [30 eyes] received adrenaline infusion at 1/1,000,000 concentration intraoperatively. Pre- and one month postoperatively, a complete ophthalmologic examination as well as endothelial evaluation with ConfoScan III was performed. Effective phaco time [EPT] and pupillary condition during the surgery were recorded. Differences between the two groups were not statistically significant in demographic characteristics, lens opacity and EPT. Endothelial cell density was 2737 +/- 321 in the bolus group vs 2742 +/- 426 in the infusion group preoperatively [P=0.1]. After one month, the rate of cell loss was 7.21% in the infusion group and 8.87% in the bolus group [P= 0.13]. Pupil diameter was >6 mm in 48% of the infusion group vs 33% of the bolus group [P=0.5]. Adrenaline was safe at the studied concentrations and there was no significant difference between bolus and infusion routes of administration in terms of effects on pupil and endothelial cells
RESUMO
To compare the effect of religious fasting on basal tear secretion [BTS], tear break up time [TBT] and intraocular pressure [IOP] in Ramadan 2005. One-hundred fifty-six healthy male volunteers less than 40 years of age from Tehran, Zahedan, Ahvaz, Mashhad and Tabriz with no ocular and systemic disease participated in this study. Weight, urine specific gravity, BTS, TBT and IOP were measured at 8:00 AM one week before as well as at 8:00 AM and 5.00 PM in the third week of Ramadan. Mean age of participants was 30 +/- 5.9 years. Duration of fasting was 12-13 hours. Mean TBT, BTS and IOP decreased by 1.8 second [P<0.0001], 2.1 mm [P<0.0001] and 0.5 mmHg [P<0.0001], respectively at 5:00 PM in the third week of Ramadan compared to 8:00 AM one week before Ramadan. IOP reduction was not clinically significant. Significant decrease in BTS and TBT was seen after three weeks of religions fasting
RESUMO
Stem cell biology has been the subject of much recent discussion. Embryonic stem [ES] cells, derived from the inner cell mass of the blastocyst stage of early mammalian embryos are expected to become a powerful tool in future regenerative medicine and developmental biology due to their capacity of selfrenewal and pluripotency. In the present study, the ultrastructural development of mouse ES cell derived cardiomyocytes was compared with invivo cardiomyocytes.. Cardiomyocytes were derived from mouse ES line [Royan B1] which developed spontaneously. The cultured cardiomyocytes were processed 3, 7, 14 and 21days after plating [day 7] for immuno histochemistry and transmission electron microscopy [TEM]. The in vivo cardiomyocytes were derived from16 days old fetuses and 2 and 8 days old pups. The beating cells expressed alpha-actinin. The maturation of the ultrastructure of cardiomyocytes depended on enhancement of development and expressed as myofibrillar bundle organization and exhibited intercalated discs, Z-disc, A, I, and H-bands, and M-line. While 7+21 days old cardiomyocytes showed all sarcomeric components such as A, I, and H-bands, Z-disc, and also M-line, T-tubule, sarcoplasmic reticulum and intercalated discs, early stage [7+3d, 7+7d and 7+14d] cardiomyocytes had few primary characteristics of subcellular structure. In fetal and 2-days old pups, the M-line was not visible. M-line was present in 8-days old pups frequently. Based on our data, mature cardiomyocytes can be produced from ES cells, and ES cell provide a good model for cardiomyocyte development. The cells can be used for cell therapy in future