[Samples for medical virology analysis]

Like all other medical biology tests, the one of medical virology start by the pre-analytical phase, which is a critical step and difficult to manage. It covers the medical prescription with the gathering of the relevant clinical and epidemiological data, the carrying out of the sampling, according to particular modalities, its transport and its storage up to the analysis site. To ensure the accuracy of results, these steps should be in accordance with regulatory requirements, especially according to the universal recognized standards. Among them, the guidance of good practice of analyzes which is the only mandatory reference, opposable to any medical laboratory and other referential taken as voluntary quality approach, for which standard of international accreditation ISO 15189, specific to medical laboratories and which combines requirements regarding quality management and technical requirements

Constitutional deficits in coagulation factors

Hereditary deficit of coagulation factors is rare affection. This is a retrospective study which analyse the coagulation parameters of 25 patients with an hereditary deficit. Deficiency on factor V, VII and VIII were the most frequent. Generally without symptoms, hereditary deficiency of coagulation factors must to be diagnosing with a great prudence for prevention of hemorrhagie riskin surgery

New resistance mechanism of escherichia coli to beta-lactamins

Escherichia coli is the germ most frequently isolated in espicially urinary infections. The perpetual change of its sensitivity profile to beta-lactamins is [due to several evolution factors. Some strains have acquiered resistance by gene mutation or by acquisition of movable genetic material. Also phenomenon as impermeability, excretion by efflux system or modifying penicillin liaison proteins could be seen but the production of beta-lactamases remains the most frequent mechanism of resistance that has been describe until now. The authors report, through this observation, a case of a particular strain of Escherichia coli that has been isoleted from urine of an hospitalized patiente. This strain presented an hyperproduction of cefalosporinases, which is a recently discovered mechanism of resistance. In one hand, specific bacteriologic tests as synergy test, antagonism test and the use of cloxacillin added antibiogramme on Mueller-Hinton medium were carried out and have allowed to display the phenotypic aspect of this mechanism. In the other hand, its genetic aspect was mentionned through a new literature data. The authors have also underlined the importance of the antibiogramme prior to any prescription of antibiotics which could put pressure on the germ leading to a selection of individual resistant, to this kind of difficul not controlled resistance and many potential complications ensue from it