RESUMO
Natural flavors are widely used in various foods, cosmetic and pharmaceutical products. These kinds of additives are applied as colors, preservatives, aroma and tasting agents. The large-scale use of certain food flavors requires accumulation of toxicological data on these substances, particularly in cases where structural similarities with other known substances showing genotoxic or carcinogenic properties indicate that some restrictions on human consumption or exposure should be implemented the case of the flavors. In this study, Concentrations of 50, 100, 200, 300, 500, 1000 and 2000 micro g/ml of four essential oils, Eugenia caryophyllata [Clove], Cinnamum zeylanicum [Cinnamon], Thymus vulgaris [Thyme] and Zataria multiflora, were tested in Salmonella typhymurium strains TA100 with and without rat liver S9 using Ames Salmonella reversion assay. Without S9 fraction, increase in mutant colonies per plate was not observed in all used concentrations. Also with S9 fraction all of samples had no significant increase in mutant colonies per plate except Clove in 500 micro g/ml, and higher concentrations. Based on obtained results, Clove oil may have mutagenic effect in 500 micro g/ml and higher concentration