Modulation of radiation and cadmium induced biochemical changes in mouse kidney by Emblica officinalis Linn

Protective effect of Emblica against radiation and cadmium induced biochemical changes in mouse kidney has been studied. Adult male mice were divided into seven groups: I [shamirradiated], II [cadmium chloride], III [irradiated with 2 Gy gamma rays], IV [radiation and cadmium chloride], V [Cadmium chloride and Emblica], VI [radiation and Emblica], VII [radiation, cadmium chloride and Emblica]. The animals were autopsied after 1-28 days of treatment. The kidney was taken out and different biochemical parameters such as total proteins, glycogen, cholesterol, acid phosphatase activity, alkaline phosphatase activity, DNA and RNA were estimated. The value of glycogen, RNA, acid phosphatase and alkaline phosphatase activity increased up to day-14 in non drug treated groups and day-7 in the Emblica treated groups and thereafter decreased up to the last autopsy interval. The value of cholesterol and DNA decreased up to day-14 in non drug treated groups and day 7 in the drug treated groups then increased in all the groups. In groups III, IV, VI and VII the value of total proteins increased during early intervals and decreased thereafter, but the animals of groups II and V, which were given only cadmium chloride with or without Emblica, showed an opposite trend. The biochemical parameters showed highly significant values [p<0.001] as compared to normal ones. Results indicated that combined treatment of radiation and cadmium chloride exerts synergistic effect. The drug treated animals showed less severe biochemical changes and an early and fast recovery, which may be due to protection provided by Emblica

Radiation and cadmium induced biochemical alterations in mouse kidney

In the present investigation radiation and cadmium induced biochemical changes in the kidney of Swiss albino mice have been studied. For this purpose, adult male Swiss albino mice [6-8 weeks old] were divided into four groups. Group I [sham-irradiated], Group II [treated with CdCl[2] solution 20 ppm], Group III [irradiated with 1.25, 2.5 and 5.0 Gy gamma rays], Group IV [both irradiated with 1.25, 2.5 and 5.0 Gy gamma rays and treated with CdCl[2] solution]. The animals were autopsied after 1, 2, 4, 7, 14 and 28 days of treatment. The kidney was taken out and different biochemical parameters, such as total proteins, glycogen, cholesterol, acid phosphatase activity, alkaline phosphatase activity, DNA and RNA were estimated. In irradiated animals, the values of total proteins, glycogen, acid phosphatase, alkaline phosphatase activity and RNA increased continuously up to day-7 and decreased thereafter up to day-28. The changes were dose dependent. In CdCl[2] treated animals, the values of glycogen and total proteins decreased during the early intervals and increased thereafter whereas the values of acid and alkaline phosphatase activity and RNA increased during early intervals and decreased thereafter. The values of cholesterol and DNA showed decrease in all the experimental groups [except group I] up to day-7 and increase thereafter up to day-28. After combined treatment also, the parameters followed the same pattern of increase and decrease, but the changes were more pronounced indicating their synergistic effect. The biochemical parameters showed highly significant values [P<0.001] as compared to normal ones. These results indicate that combined treatment of cadmium and gamma radiations causes synergistic or additive effect