RESUMO
Objective: To detect the anti cyclic citrullinated peptide [Anti-CCP] antibody in rheumatoid arthritis [RA] patients to determine its diagnostic value in Pakistani patients
Study Design: Cross sectional study
Place and Duration of Study: Military Hospital [MH] Rawalpindi, from January 2013 to June 2015
Material and Methods: A total of 58 patients with complications of rheumatoid arthritis were recruited in the study using convenient sampling technique after their informed consent. Age and gender of the patients were recorded. Blood was collected from the patients subjected to ELISA based detection of anti-CCP and latex agglutination test for detection of Rheumatoid Factor [RF]. Data obtained were analyzed using Microsoft excel 2010
Results: Among the fifty eight RA patients, 40% were males and 60% were females. Age ranged between 12 to 80 years [mean age 49.74 +/- 16.81 years] of the males RA patients and was higher as compared to females [mean age 43.2 +/- 16.70 years]. ELISA based detection of anti-CCP antibody showed that about 91 percent of RA patients were positive for anti CCP antibody. About 72% were positive for anti CCP antibody alone, 19 percent were positive for both anti-CCP and RF and 9 percent were positive for RF
Conclusion: The results concluded that a higher percentage of the RA patients are positive for anti-CCP antibody marking its importance as a diagnostic marker. Anti-CCP has more sensitivity as compared to RF in RA patients
RESUMO
Pancytopenia is a reduction in the number of each type of peripheral blood cell. Therefore the role of bone marrow examintion in diagnosis of pancytopenia is important to know etiology of pancytopenia. The objective of the study was to know the aetiology of pancytopenia. This descriptive [Cross sectional] study was carried out in Khyber teaching hospital. Fifty patients with pancytopenia were included in the study from 1[st] January 2008 to 30[th] October 2008. Full blood counts, bone marrow examinations and trephine biopsies were performed according to standard methods. Statistical packages for social science [SPSS.11] was used to analyze data. Out of 50 patients, 36% were of aplastic anaemia, 16% megaloblastic anaemia, 14% myelodysplastic syndrome and 12% acute lymphoblastic leukemia [ALL], Hypersplenism in 10%, 4% non Hodgkin lymphoma [NHL] and 4% multiple myeloma, 2% each of acute myeloblastic leukemia and chronic myelocytic leukemia. All of these disorders were common in male as compared to female. Aplastic anaemia was the commonest cause of pancytopenia followed by megaloblastic anemia and myelodysplastic syndrome in our study