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Introduction. Monos Group, Drug Research Institute is starting to investigate of Ellipin preparationfrom the mid-1990s, Ellipin has anti cancer activity in liver and several studies were investigated withscientists from Japan and China. Especially Hayashi K., Khurelbaatar L and Ambaga M were determinedanti-cancer action of the preparation and they were explained of mechanism of action, which apoptosisis seduced by influence of unsaturated fatty acids in tumor cells. However, changes of fatty acidscomposition at production stage were did not study yet. Therefore, we studied that composition of fattyacids in different term of production stage and compared of Ellipin dense substance.Materials and Methods. Samples of study were collected from production stage of “Ellipin” series130304, which was tacked in 48th hour, 120th hour of production. Each sample was dried at freezedryer “Labconco freezone12L” in Drug Research Institute. Total lipids of sample were extracted withchloroform: methanol (2: 1 v/v) according to Folch et al. Fatty acid methyl esters were analyzed usingAgilent Packard Gas Chromatograph (GC) (Model HP-6890 Agilent Packard) with mass-spectrumdetector (Model HP MSD 5973N) of Buryat State University, in Ulan-Ude.Results. Ellipin preparation is derived from bovine liver, and which is based on homogenization of bovineliver for isotonic. In this process, unsaturated fatty acids were extracted in organic solution. We studiedchanges which saturated and unsaturated fatty acids of bovine liver in process of homogenization andconsist of each fatty acid contents of end product. Results have shown that unsaturated fatty acidswere decreased by 0.4-44% till 120th hour of homogenization process. While, there were decreasedby 4-12% in the end product, although, ω-6 fatty acids were increased by 13.1-38.4%. Moreover, 25saturated fatty acids and 12 unsaturated fatty acids were detected in the Ellipin dense substance (endproduct). Hence, 67.5% of total fatty acid was saturated fatty acids, 32.5% was unsaturated fatty acidsin the Ellipin dense substance. Resent results and results of previous studies indicated that Ellipindense substance may contains saturated fatty acids on in average 50.34%, unsaturated fatty acids onin average 49,32%, respectively.Conclusion. Proportion of saturated and unsaturated fatty acids in Ellipin production was about 2:1.Saturated fatty acids and unsaturated fatty acids were found 25 and 12, respectively. Saturated fattyacids were gradually decreased and unsaturated fatty acids were slowly increased in production period,which from 48th hour of production-conveyer till end product. Moreover, content of ω-3-6-9 fatty acidswas consist 83,9-87,5% of total unsaturated fatty acid.
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Topicality: In recent years interest to biologically active preparations of a phytogenesis is considerably increased. Tannins are very widespread group of biologically active agents of the plants, having various pharmacological properties (astringent, styp tic, antiinflammatory), and causing their broad application in m edicine.[1] Therefore the problem of determination of high quality of medicines and the medicinal plant raw materials containing this group of biologically active agents is very actual. The techniques, allowing to establish high quality of the raw materials containing tannins, were applied to raw materials of Rhizomata Bergeniae crassifoliae from an original derivative plant of a Bergenia crassifolia (L. ) Fritsch, genus of Bergenia. The purpose of this work: is to carry out a number of research works on studying of a chemical composition of the medicinal plant raw materials containing tannins, and to make a precise characteristic of these plants on the basis of the received results. According to a goal of this research the following tasks are defined: 1. To carry out the macroscopic and microscopical analysis of medicinal raw materials of Rhizomata Bergeniae crassifoliae 2. To define the main indicators of commodity research;3. To define quality and quantity biologically active agents in these plants. For the solution of tasks the following Methods: of the analysis were used:macroscopic, microscopical, phytochemicaland histochemical analysis, thin-layerchromatography and spectophotometery.Powder of Rhizomata Bergeniae crassifoliae(Krasnogorseleksredstva ltd. production) and crumbled of Rhizomata Bergeniae crassifoliae (the manufacturer of Ivan-Chay ltd.) were used as samples for this analysis.Results: According to pharmacopeia article “ Rhizomata Bergeniae crassifoliae “, for the specified raw materials are established the following numerical indicators: quantity tannins not less than 20%; humidity no more than 14%; total ash no more than 4%; ashes, insoluble in 10% Acidum hydrochloricum solution, no more than 0,5%. [2] The analyzed raw materials correspond to these indicators. As a result of carrying out histochemical and phytochemical reactions in rhizomes of a bergenia the following biologically active agents were found: arbutin, tannins (catechol, EGCG), polysaccharides.Conclusion: Medicinal plant raw materials ofKrasnogorseleksredstva ltd. and Ivan-Chay ltd.correspond to the general pharmacopeia article“Rhizomata Bergeniae crassifoliae”.References1. L.M. Fedoseeva. Study of tannins and aboveground vegetative organs Bergeniae crassifoliae (L. ) Fritsch native of Altai / L.M. Fedoseeva, E.V. Timochin // Chemistry of plant raw material. – 2003. - №1. – pp.73-77.2. State pharmacopeia Ed. XI. M.: Medicine, 1990. T.2. p. 399.3. State pharmacopeia Ed. XI. M.: Medicine, 1987. T.1. p. 335.4. G.P. Yakovlev. Rhizomata Bergeniae crassifoliae / G.P. Yakovlev, I.A. Samilina, L.V. Moshkova // Pharmacognozy. SPb.: SpecLit, 2006. pp. 556-558.5. I.M. Korenskaya. Qualitative and quantitativeanalysis of medicinal plant raw materials containing tannins. / I.M. Korenskaya, N.P. Ivanovskaya, I.E. Izmalkova // Medicinal plants and medicinal plant raw materials containing simple phenols, lignans, tannins. Voronezh, 2007. Pp. 48-51Methods: of the analysis were used: macroscopic, microscopical, phytochemical and histochemical analysis, thin laye r chromatography and
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Topicality. Class polysaccharides take up a important position among biological active substances. Representatives of this class are compounds which have broad spectrum pharmacological properties. They are under in sphere attention of researchers. Tussilago farfarae L. (Asteracea) concerm to such plant. These is plants are grown in Europe and Asia. It is also a common plant in North America and South America where it has been introducted, most likely by settlers as a medical item. The plant is often found in waste and disturbed places and along roadsides and paths. In some areas it is considered an invasive species [1].The purpose of this work is to pharmacognostical analysis Folia Farfarae. According to a goal of this research the following tasks are defined:1. To carry out the macroscopic and microscopic analysis of materials.2. To indentify major merchandising performances.3. To define the amount of active ingredients on whichthe raw materials are standardized.As samples for the analysis served: Folia Farfarae officinalis by “Ivan-Chai” were served as samples for the analysis.For the solution of tasks the following methods of analysis were used: macroscopic,macroscopycal, phytochemical, histochemical, gravimetric analysis.Results. According to pharmacopeia article “Folia Farfarae” (State Pharmacopeia Ed. XI,T.2,p.280), for the specified raw materials are established the following numeral indicators: Ashes in general (no more than 20%); ashes, nsoluble in 10% HCL (no more than 10%); humidity (no more than 13%) [2].Conclusion: “Folia Farfarae” correspond to the general pharmacopeia article “Folia Farfarae”.References:1. Yakovleva G.P., Blinova K.F. “Medical plant raw materials. Pharmacognosy” // special literature,2004. pp.77-78 (on russian).2. State Pharmacopeia of USSR Ed. XI,T.2, p.280 “Folia Farfarae
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Topicaliti: Melissa officinalis L.- a perennial essential oil plant, a sort of the Melissa (Lamiaceae) [1] Plants were used as medicines in the ancient time and are still successfully applied to treat various diseases are used and in the development of new phytopreparations. Herbal medicinal product in comparison with synthetic, have such advantages as low cost of raw materials, less side effects, softer impact on organism and availability. Essential oils of plants are a source of biologically active agents on the basis of which creation of new medicines is possible: «casmin» anticoagulant, anti- aggregative collection «Lomagerpan» cream (contains dry extract from Herba Melissa officinalis). In the world there are 2500 species of such plants, 450 of them grow in Kazakhstan, in the Mediterranean area in the countries of the Middle East. There are medicinal Herba Melissa officinalis.The purpose of this work: is to study PharmacognosticHerba Melissa officinalisAccording to goal of this research the following tasksare defined:1) To carry out the macroscopic and microscopic analysis of materials.2) Identify major merchandising performance.To define the amount of active ingredients on whichthe raw materials are standardized.As samples for the analysis served: Herba Melissa officinalis by «Zdorovye» were served as samples for the analysis. For achievement of objectives the following methods were used: the macro - and the microscopic analysis, the phytochemical analysis, a refraction-refractometry indicator. [2]Results: According to Pharmacopian article ¹ 42- 3645-98 (of Russia) [3] numerical indicators for a Herba Melissa officinalis following: the sum of the extractive substances drawn of 24% by alcohol (not less than 22 %), humidity (no more than 12 %); ashes of the general (no more than 12 %); ashes insoluble in 10% solution of acid hydrochloric (no more than 3 %); not passing through a sieve with openings with a diameter of 7 mm, (no more than 17 %); organic impurity (no more than 2%); mineral impurity (no more than 1 %).Conclusion: Herba Melissa officinalis conform to the pharmacopein article 42-3645-98Referenses:1 Yakovleva G.P, Blinova K.F. «Medicinal plant raw materials Pharmacognosy»//special Literature, 2004. pp.305-308 (on russian)2 State Pharmacopeia of USSR issue 1 pp.29-303 Pharmacopeia article 42-3645-98 literature (on russian) Herba Melissa officinalis