Development and comparision of an HPTLC, HPLC and LC-MS method for determinationof tetracyline antibiotics.

Tetracycline (TC) antibiotics continue to play an important role in human and veterinary medicine and in animal nutrition. Comparison of three techniques, High Performance thin-layer chromatographic (HPTLC), HPLC and LC-MSMS for the determination of tetracycline has been carried out here. The purpose of this study was to determination of minimum responce tetracyclines and their metabolite by chromatographic techniqes. A rapid and easy‐to‐use method was developed on the liquid chromatography.