In vitro Cytotoxic Activity of New Triphenyltin (IV) Alkyl-isopropyl-di-thiocarbamate Compounds on Human Acute T-Lymphoblastic Cell Line.

Cancer is one of the leading causes of mortality and morbidity worldwide. Various new metal-based compounds including organotin (IV) compounds have been actively synthesised due to their good cytotoxicity in cancerous cells. In this study, we tested the cytotoxicity of new triphenyltin (IV) dithiocarbamate compounds (triphenyltin (IV) benzylisopropyldithiocarbamate, compound 1; triphenyltin (IV) methylisopropyldithiocarbamate, compound 2; and triphenyltin (IV) ethylisopropyldithiocarbamate, compound 3) on the human acute T-lymphoblastic cell line, Jurkat E6.1 by MTT assay at three periods of time. The triphenyltin (IV) methylisopropyldithiocarbamate compound showed the lowest IC50 values (0.03 μM) after 24 h of treatment on Jurkat E6.1 cell lines. The IC50 values obtained for the three compounds for 24 h of treatment were 0.18 μM, 0.03 μM, and 0.42 μM, respectively. Next, for 48 h and 72 h of treatment, the IC50 values were 0.15 μM, 0.18 μM, and 0.40 μM and 0.18 μM, 0.19 μM, and 0.41 μM, respectively. These tested compounds were found to give cytotoxic activity against Jurkat E6.1 cell lines at micromolar doses. Observation on morphological changes of Jurkat E6.1 cell lines treated with IC50 values at 24 h of treatment showed morphological changes such as cell shrinkage and membrane blebbing, characterising the mode of cell death as apoptosis. Thus, further studies on the specific mechanisms of action of these compounds in human cells should be carried out to elucidate their potential as anticancer agents.

Clarias batrachus collagen extract increases fibroblast cell adhesion, migration and proliferation.

Collagen is an extracellular matrix protein with great importance in biomedical application. The search for collagen from various sources is intensified especially from marine source. This study was carried out to extract collagen from a Malaysian local fresh water fish, Clarias batrachus and characterized its biomedical potential In vitro. Collagen was extracted from C. batrachus skin using acetic acid method and identified using SDS-PAGE. MTT assay was performed to determine the effect of coated collagen on cell adhesion and proliferation of L929 skin fibroblast cells. Additionally, scratch assay was performed to examine the effect of collagen coating on fibroblast cell migration. Result showed that collagen extracted from C. batrachus was made up of collagen type I, which consists of two α chains (α-1 and α-2) and β chain. At 100 μg/cm2 density, collagen coating significantly increased fibroblast cell adhesion, proliferation and migration compared to negative control (p< 0.05). As a conclusion, collagen extracted from C. batrachus increased cell adhesion, proliferation and migration of fibroblast cells and has potential to be used as an alternative source of collagen.