RESUMO
Background: it is reasonable to think that some biochemical characteristics of follicular fluid [FF] surrounding the oocyte may play a critical role in determining the quality of oocyte and the subsequent potential needed to achieve fertilization and embryo development
Objective: this study was carried out to evaluate the levels of FF homocysteine [Hcy] in IVF candidate polycystic ovary syndrome [PCOS] women and any relationships with FF glucose and estradiol [E[2]] levels
Materials and Methods: in this case control study which was performed in Dr. Tizro Day Care and IVF Center 70 infertile patients were enrolled in two groups: comprising 35 PCOS and 35 non PCOS women. Long protocol was performed for all patients. FF Hcy, glucose and E[2] levels were analyzed at the time of oocyte retrieval
Results: it was observed that FF Hcy level was significantly higher in PCOS patients compared with non PCOSs [p<0.01]. Observations demonstrated that in PCOS group, the Hcy level increased independent to E[2], glucose levels, BMI and age, while the PCOS group showed significantly higher BMI compared with non-PCOS group [p=0.03]. However, no significant differences were revealed between groups for FF glucose and E[2] levels
Conclusion: present data showed that although FF glucose and E[2] levels were constant in PCOS and non PCOS patients, but the FF Hcy levels in PCOS were significantly increased [p=0.01]
RESUMO
This research studied the effect of ciprofloxacin [CPFX] on spermatogenesis. We aimed to estimate the effect of CPFX on serum levels of testosterone, LH and FSH. In this experimental study, a total of 24 mice were assigned to controlsham and test groups. We subdivided the test group into low [206 mg/kg] and high [412 mg/kg] dose CPFX groups. Control-sham animals received carboxymethyl cellulose [CMC]. All animals were treated orally for 45 days. Cytoplasmic carbohydrate, lipid accumulation, cytoplasmic lipase and alkaline phosphatase [ALP] ratios were examined. Serum levels of luteinizing hormone [LH], follicle stimulating hormone [FSH ] and testosterone were measured in the control and test groups. The spermatogenesis cell series exhibited low numbers of cells with periodic acid Schiff [PAS]-positive cytoplasm and higher numbers of cells with lipid-positive foci. The tissue to ALP ratio and germinal epithelium [GE] lipase synthesis increased in CPFX-treated animals. In contrast to the CPFX groups, control animals showed normal cytoplasmic carbohydrate, lipid, lipase and ALP ratios in all cellular layers. In the CPFX-treated groups there was a significantly lower serum testosterone level compared with the control group. The serum levels of FSH and LH in high dosetreated animals decreased. Our results suggest that following long time CPFX administration major alterations occur in GE intracytoplasmic biochemistry, which may lead to loss of physiological function and ultimately result in fertility problems. CPFX is able to imbalance serum levels of gonadotropins and testosterone levels by affecting Leydig cells