Diabetes and experimental pregnancy in rats: course of maternal blood glucose levels and its repercussions on the blood glucose levels and pancreas of newborn pups

The objective of the present investigation was to determine the course of maternal blood glucose levels in pregnant rats and its repercussions on the glucose levels an pancreas of their newborn pups. Diabetes was induced by alloxan (42mg/Kg body weight) and steptozotocin (40mg/Kg). Sixty-two pregnant Wistar rats weighing 180 to 250 g were divided into a control group and two groups with moderate (120 to 200 mg/dl glucose) and severe diabetes (greater than 200 mg/dl glucose), respectively. Blood glucose levels were measured in the dams on the 1st, 14th, and 21st days of pregnancy and in the pups at birth. The results were pooled for each litter. The fetal pancrases were removed after cesarian section performed on the 21st day of pregnancy, pooled for each litter and processed for histopathologic examination by light microscopy. Maternal blood glucose levels were significantly increased compared with the first day of pregnancy in both normal and diabetic ratsd starting on the 14 th day of pregnancy. Fetal blood glucose levels correlated with maternal levels. The histopathologic changes characterized by vacuolization and basophilia of the cytoplasm of endocrine pancreas of newborn pups from dams with moderate or severe diabetes suggested pancreatic hyperactivity

A origem do hirsutismo: avaliacao dos niveis de sulfato de deidroepiandrosterona, prolactina e hormonio luteinizante. / The origin of hirsutism: evaluation of dehidroepiandrosterone sulfate, prolactin and luteinizing hormone

A producao excessiva de androgenios da supra-renal, induzindo a um subsequente aumento de androgenios ovarianos, constitui um provavel mecanismo envolvido na forma mista de hirsutismo. O fenomeno inverso ou seja, a alteracao inicialmente ovariana, levando a uma subsequente alteracao supra-renal, nao e claro no momento. Com o objetivo de estudar essa possibilidade, foram dosados por metodos radioimunologicos os niveis plasmaticos do sulfato de deidroepiandrosterona (DHEA-S), prolactina (PRL) e hormonio luteinizante (LH) em 12 pacientes hirsutas eumenorreicas (grupo I) e em 12 pacientes hirsutas oligmomenorreicas (grupo II). As portadoras de ciclos irregulares apresentaram niveis significantes mais altos de LH e PRL. Quanto ao DHEA-S, nao houve diferenca entre os grupos. Nas pacientes do grupo II, o aumento de LH parece indicar uma maior frequencia de uma participacao ovariana no processo.Sendo a PRL capaz de estimular a supra-renal, a sua elevacao entre as pacientes oligomenorreicas poderia explicar a possibilidade de um hirsutismo inicialmente de origem ovariana se transformar em uma forma mista