[Double negative T cells correlate with disease activity in Systemic Lupus Erythematosus patients]

Systemic lupus erythematosus [SLE] is a complex multisystem autoimmune disease. It is characterized by multisystem affection with remission and relapse course. T lymphocytes characterized as TCR alpha beta CD4[-] CDS8[-] CD56- cells are known as `double-negative' [DN] T cells and have been described in both human and rodent models. The present study included thirty patients with SLE and sixteen healthy blood donor females. All cases and controls subjected to clinical assessment and DNT cells percentage measurement in periephral blood mononuclear cells [PBMNCs]. Our findings suggest that DN T cells subset; appear to play an essential role in SLE as it was correlated with disease, activity

Effect of major burn on immunophenotype of peripheral blood T lymphocytes

The immunosuppressive effect of a major burn has been known for many years. However, a complete understanding of the effects of a burn on the immune system remains elusive. Lymphocytes immunophenotype is a reflection of the functional level of immune system. There is little knowledge concerning the expression of HLA-DR on peripheral blood [Pb] T lymphocytes. T lymphocytes of 26 major burn [25-40%] patients were analyzed in 24 hours, 1 week and 2 weeks after burn, using, monoclonal antibodies of CD3, CD4, CD8, CD25 [IL2R,] and HLA-DR by flow cytometry and comparing them with those of26 apparently healthy donors. There was statistically significant reduction in absolute number of CD3 [p<0.0001], CD4/CD8 ratio [p=0.01] in the first 24 h in comparison with controls. CD25 [IL2R] shows insignificant upregulation on T lymphocytes after burn with significant upregulation of HLA-DR. The absolute number of CD3[+] cells began to increase after 2 weeks [p=0.03]. but still reduced than controls [p=0. 08,]. CD4/CD8 ratio was more or less as healthy control after 2 weeks. Upregulation of CD25 was insignificantly increased and that of HLA-DR were marked increased after 2 weeks. The absolute number CD25 and HLA-DR[+] T lymphocyte subsets all over the time of the study are low than controls except that of HLA-DR[+] T lymphocytes after 2 weeks [p=0.009]. The data obtained suggest persistent activation of T lymphocytes 2 weeks post major burns. HLA-DR expression can reflect post burn lymphocyte activation