RESUMO
Objective: To evaluate effects of pyrroloquinoline quinone [PQQ] on ischemia-reperfusion injury using a rat ovary model
Method: Thirty healthy female Wistar rats with 250g were randomized into five experimental groups [n = 6]: Group SHAM: The rats underwent only laparotomy. Group Ischemia: A 3- hour ischemia only. Group I/R: A 3-hour ischemia and a 3-hour reperfusion. 30 min before termination of reperfusion 20 microL soybeen oil [Solvent of PQQ] was administered. Group I/PQQ: A 3-hour ischemia only and 20 microL [10 mg/kg] intraperitoneal administration [IP] of PQQ 2.5 hours after induction of ischemia. Group I/R/PQQ: A 3-hour ischemia, a 3-hour reperfusion and 20 microL [10 mg/kg] IP of PQQ 2.5 hours after induction of ischemia
Results: Animals treated with PQQ showed significantly ameliorated development of ischemia and reperfusion tissue injury compared to those of other groups [p=0.001]. The significant higher values of SOD, GPO and GST were observed in I/R/PQQ animals compared to those of other groups [p=0.001]. Damage indicator [MDA] was significantly lower in I/R/PQQ animal compared to those of other groups [p=0.001]
Conclusion: Intraperitoneal administration of PQQ could be helpful in minimizing ischemia-reperfusion injury in ovarian tissue exposed to ischemia