Determination of resource mobilization during seedling growth of palmyra palm, Borassus flabellifer L

Asian palmyra palm (Borassus flabellifer L.) is a multipurpose tree with year round products as food for the dependent society. Besides edible items the tree offers around 801 useful goods which are economically important. Such a plant’s seedling biology has not been fully understood. Hence, the present study was aimed at understanding seedling developmental stages and resource mobilisation in Palmyra palm and further to determine the role of cotyledonary sheath (CS) during entire seedling growth. We investigated the developmental changes throughout growth of seedlings by providing different sets of growth conditions. Artificial seed bed made of coconut coir was compared with that of soil conditions to study germination and establishment of seedlings. Phloem loading dye was used to track the flow of nutrients from embryo to cotyledonary sheath. Seed germination in palmyra palm is hypogeal by forming ligular and tubular structures remotely in soil. Eight new organs differentiate from the seed embryo that includes haustorium, ligule, cotyledonary sheath, cataphyll, eophyll, mesocotyle (junction), primary root and mesocotyl roots during seed germination. Among these the first four are temporary organs and they disintegrate once the seedling is well established. The last four organs are responsible for developing acomplete plantlet at later stages. The seed and seedling organs of Palmyra palm have four major storage reserves to support successful germination and firm establishment of seedling. Palmyra has evolved to control the solubilisation, movement and regulation of food among transient seedling organs and carry out translocation of food to the developing and differentiating organs. Seedlings also have developed physiological functions and strategies to mobilise the stored food without losing them at any point of their growth and developmental stages. Water required for seed germination permeates laterally only viacotyledonary sheath which has spongy tissues and lenticels all over. These tissues are the primary mode of water supply as the seedlings lack major root organs in the early stages of development. Flourescent microscopic and anatomical studies were carried out to observe the transport and storage of food substances required during seedling growth. Histochemical studies of seedling organs have revealed the presence of various type of nutrients such as simple sugars, carbohydrates, proteins, amino acids and lipids.

Hematological changes following early ligation of splenic artery during splenectomy in shunt surgery for portal hypertension.

Objective and background data: Reduction in cellular elements of blood, secondary to hypersplenism is an established component of non-cirrhotic portal hypertension. Prior transfusion of blood or blood components is frequently required for safe surgical intervention. Due to thrombocytopenia, epidural catheter insertion for effective and durable analgesia is not possible. The aim of the present study was to objectively demonstrate the gain in blood components following early ligation of splenic artery for splenectomy in shunt surgery. Methods: From Jan 2008 to July 2010, 30 patients underwent elective proximal spleno renal shunt for portal hypertension, for various indications and were analyzed prospectively. We followed the standard protocol of ligating the splenic artery in situ, first in the lesser sac. Proximal spleno shunt was done . After the surgical procedure and before extubation, an epidural catheter was placed for effective and durable analgesia. 5ml of venous blood was drawn in the following order of sequence: prior to induction of anesthesia, immediately after the ligation of splenic artery, 30 minutes after ligation of splenic artery and 30 minutes after splenectomy. Samples were sent for complete hemogram and values were analyzed in respective order. Patients requiring transfusion of blood or blood components during surgery were excluded from the study. Results: 30 patients (M - 9, F- 21) with mean age of 29.4 years ( 11-60 years) were analyzed (NCPF- 20, EHPVO- 9, cirrhosis- 1). We objectively demonstrated a significant gain in RBCs (p=0.016) and platelets (p=0.000) using this standard protocol. As there were no intrinsic abnormalities in RBCs, red blood cell indices (MCV, MCH, MCHC) showed no changes as expected (p-0.9). Conclusion: By following this standard protocol, in addition to reduction in blood loss there was a significant gain in RBCs and platelets. This gain allows the surgeon to perform the surgical procedure safely and the anesthetist to secure an epidural catheter immediately after surgery for effective and durable analgesia without prior transfusion.

In vitro adventitious shoot formation from leaf cultures of Clerodendrum inerme (L) Gaertn.

In vitro adventitious shoots (about 28) of Clerodendrum inerme were regenerated from leaf segments on MS medium containing BA (4 mg/L). These shoots developed directly from the leaf explants without callusing after 5 weeks. Leaf explant when cultured in MS medium containing BA (2 mg/L) and NAA (0.5 mg/L) developed compact callus that became nodular and regenerated shoots (about 50) after 5 weeks. The in vitro developed shoots were rooted in MS medium supplemented with IAA (2 mg/L). The hardened plantlets were successfully established in the field with 90% survival.

Delinquency in children.

Delinquency among children is not a problem only in Western countries. Psychosocial analysis of inmates of an approved school revealed that broken home was the reason for the emotional stress which triggered antisocial behaviour. Children want to be with their parents inspite of the injustice done to them.