RESUMO
<p><b>OBJECTIVE</b>To develop a method for the determination of Chonglou saponin I and Chonglou saponin II in Yifu ointment.</p><p><b>METHOD</b>The chromatographic separation was performed on Hypersil ODS2 C18 column (4.6 mm x 150 mm, 5 microm) and IBBM-612111 ODS C18 guard column. Acetonitrile-0.02% phosphoric (43:57) as mobile phase. The flow rate was 1 mL min(-1) and column temperature was set at 40 degrees C. The UV detection wavelength was set 203 nm.</p><p><b>RESULT</b>Chonglou saponin I showed a good linear relationship at a range of 0.1024-3.2 microg, r =0.9998, the average recovery was 97.4%, and RSD was 1.8% (n = 6); Chonglou saponin II showed a good linear relationship at a range of 0.064-2.0 microg, r = 0.9999, the average recovery was 101.4%, and RSD was 1.0% (n =6).</p><p><b>CONCLUSION</b>The method is accurate with the good reproducibility and can be used for the quality control of Yifu Ointment.</p>