Sensitive, resistant and multi-drug resistant Acinetobacter baumanii at Saudi Arabia hospital eastern region

Abstract: Since the Physicians start use of antibiotics long ago with un-notice drug resistance. However actual problem was recognized about 85 years ago. Antibiotic resistant and Multi-drug resistant bacterial strains are at rise throughout the world. It is physicians and researchers to take scientific research based appropriate action to overcome this ever-spreading problem.. This study is designed to find out sensitive [S], resistant [R] and multi-drug resistant [MDR] Acinetobacter baumanii strain along with other isolates in the resident patients of Eastern Region of Saudi Arabia. Pseudomonas aeruginosa is excluded from other gram-negative organisms isolated from different sites as it will be dealt separately. This study is based in was retrospective observations designed to collect data of different stains of Acinetobacter baumanii with reference to their Sensitivity [S], Resistance [R], Multi-Drug Resistance [MDR] along with other Gram negative isolated from different sites [from 1st January 2004 to 31st December 2011] at King Abdulaziz Hospital located Eastern Region of Kingdom of Saudi Arabia [KSA]. All necessary techniques were used to culture and perform sensitivity of these isolates. There were 4532 isolates out of which 3018 [67%] were from patients. Out of Acinetobacter baumanii infected were 906 [20%] while other 3626 [80%] isolates were miscellaneous. Numbers of patients or cases were 480 [53%] out of 906 isolates and numbers of patients or cases in other organisms were 2538 [70%] out of 3626 isolates. Acinetobacter baumanii infected patients 221 [46%] were male and 259 [54%] were female and the male and female ratio of 1:1.2. In other organisms this male female ratio was almost same. There was steady rise in number of patients and the hence the isolates from 2004 to 2011. Majority of the bacterial strains were isolated as single organism but some were isolated as double or triple or quadruple or more organisms from different sites. Sensitive, Resistant and Multi-Drug Resistant Acinetobacter baumanii have been isolated from different sites. The other Gram negative isolates included Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris, Klebsiella oxytoca, Serratia marcescens and Stenotrophomonas maltophilia. A significant rise in R and MDR but there is rise in R and MDR Acinetobacter baumanii Strains has been interceded other isolates. It is important to adopt proper and sustainable policies and guideline regarding antibiotics prescription and used. We should also check our infection control practices in our hospital or healthcare settings. We should start antibiotics stewardship in our hospital in order to reducing or overcoming antibiotics Resistant [R] and Multi-Drug Resistant [MDR] strains prevalence.

Serum leptin levels in obese infertile men and women

The present study was designed to evaluate the relationship between serum leptin and infertility in obese young and old men and women. The groups of infertile obese men [n=66] and women [n=30] compared with control obese fertile men [n=60] and women [n=30] with same ages to find the contribution of serum leptin level in causation of fertility. The results revealed that serum leptin were significantly raised in infertile male and female with p<0.001. BMI was also found to be significantly higher [p<0.001] in infertile men and women. Moreover a strong positive correlation was found between BMI and leptin level, and serum leptin and age in both fertile and infertile men and women. The values of correlation coefficients between serum leptin and BMI, and serum leptin and age are statistically significant [r=0.3-0.6, p<0.01-p<0.05]. This study has concluded that obesity is associated with infertility in men and women. Sex hormonal imbalance may also be associated BMI and serum leptin in infertility. However further studies are required to determine the exact match by which enhanced BMI and serum leptin levels to female and male infertility

Dose related effects of Buspirone on serum electrolyte balance, plasma osmolality and systolic blood pressure [SBP] in rats

Buspirone is a potent anxiolytic that decreases serotonin transmission. Changes in electrolyte balance, plasma osmolality and systolic blood pressure are often associated with stress-induced anxiety in rats as well as in human but effects of buspirone on changes in serum electrolytes balance, plasma osmolality and SBP of rats has not been reported. Present study concerns the effects of different doses of buspirone [0.25, 0.5, and 1mg/kg] on serum electrolyte, plasma osmolality and systolic blood pressure [SBP] of rats. Anxiolysis related variable are also monitored. Results show that the administration of buspirone [0.25mg/kg and 0.5mg/kg] significantly increased the serum concentration of electrolytes and plasma osmolality but decreased the serum level of magnesium. These doses also reduced the systolic blood pressure [SBP]. A dose of 1mg/kg buspirone produced no effect on the concentration of serum electrolytes, and plasma osmolality. Anxiolytic effects of the drug were dose dependent but 1mg/kg dose decreased the effect. The results are discussed in the context of serotonin receptors [5-HT[1A] to be involved in buspirone-induced changes of electrolytes, SBP and plasma osmolality

Effects of diazepam on serum electrolytes,osmolarity and anxiolysis of stressed rats

Aim of this study was to determine the effect of diazepam on serum electrolytes [sodium, potassium, calcium, and magnesium], osmolarity and anxiolysis of 2hrs restraint stressed rats. Male Albino Wistar rats were used in experiment. Test animals were injected diazepam intrap-eritoneal and control deionized water. After thirty minutes of injection control and test animals were further divided into restrained and unrestrained groups. Animals of unrestrained group were kept in home cages, while restrained grouped animals were restrained by immobilizing for two hours [2hrs] on wire grids. Next day light-dark box activity was monitored for 5 minutes. Rats were decapitated. Blood was collected and serum separated for analysis of electrolytes. Results showed that diazepam increases the serum sodium, potassium, calcium, concentration significantly [p<0.01]. It also increases the osmolarity; number of events and time spent in light box but decreased magnesium concentration significantly [P<0.01] in stressed and unstressed rats. It is concluded that the diazepam reverse the stressed produced changes in serum electrolytes and plasma osmolarity

Effect of restraint street on serum electrolytes; in rats

Aim of this study was to determine the effect of immobilization stress on serum electrolytes [sodium, potassium, calcium and magnesium] in rats. Male albino wistar rats were used in experiment. Test animals were restrained by immobilizing for two hours on wire grids. Control animals were kept unrestrained in home cages. Rats were killed after the termination of restraint period. Blood was collected and serum separated for the analysis of electrolytes. Serum sodium, potassium and calcium were analyzed by flame photometer. Magnesium content was determined by spectrophotometer. Result shows a significant decreased [P = 0.001] in sodium, potassium, and calcium but increased [P = 0.001] in magnesium serum level. It is concluded that stress induced decrease in sodium, potassium and calcium and increase in serum magnesium level may contribute to stress induce hypertension