Adiponectin, anthropometric measurements and insulin resistance in adolescence with obesity

@#Introduction: Obesity in adolescents can cause metabolic syndrome. Insulin resistance increases the risk of metabolic syndrome, which then increases the risk of premature death. Studies about anthropometric measurements and adiponectin levels as early markers of insulin resistance in obese adolescents are still limited. Methods: A cross-sectional study was performed on 59 obese adolescents aged 13–16 years. Obesity was established on the basis of the Centers for Disease Control and Prevention (CDC) curve (2000). Insulin and blood glucose level measurements were carried out using an enzymatic kit. Adiponectin levels were assayed using enzyme-linked immunosorbent assay (ELISA). The relationships between variables were evaluated by correlation analysis using SPSS. Results: Statistical tests showed a positive correlation between waist circumference (r=0.421; p=0.001) and Homeostatic Model Assessment of Insulin Resistance (HOMA-IR) (r=0.396; p=0.002). Waist-to-hip ratio (WHR) and waist-to-height ratio (WHtR) had a weak positive correlation with insulin (r=0.343; p=0.008 and r=0.311; p=0.017) and HOMA-IR (r=0.306; p=0.018). There was a weak negative correlation between adiponectin and insulin in obese adolescents (r=-0.278; p=0.033). Conclusion: Anthropometric measurements (waist circumference, WHR and WHtR) and adiponectin can be used for early detection of insulin resistance and hyperinsulinemia in obese adolescents.

Searching for the best agarose candidate from genus Gracilaria, Eucheuma, Gelidium and local brands

Objective: To explore the potential of local agar of genus Gracilaria, Eucheuma, Gelidium and local brands as an alternative for imported agarose for DNA electrophoresis, and to examine their ability related to separation and migration of DNA fragments in DNA electrophoresis. Methods: Their performance at various concentrations were compared via an experimental study with a specific brand of imported commercial agarose used in molecular biology research. The measured variables were separation and migration during electrophoresis of a DNA fragment. Results: The local agar genus Gracilaria gigas, Gelidium, brand "B" and brand "S" could separate DNA fragments at a concentration between 1% and 2%, with an optimum concentration of 2% w/v, as good as a specific brand of imported commercial agarose. Conclusions: Their performance were very close to that of commercial agarose and can still be improved by further agar purification as well as by pH and sulfur control.

Searching for the best agarose candidate from genusGracilaria,Eucheuma,Gelidium and local brands

Objective:To explore the potential of local agar of genusGracilaria,Eucheuma,Gelidium and local brandsas an alternative for imported agarose forDNA electrophoresis, and to examine their ability related to separation and migration ofDNA fragments inDNA electrophoresis. Methods:Their performance at various concentrations were compared via an experimental study with a specific brand of imported commercial agarose used in molecular biology research. The measured variables were separation and migration during electrophoresis of a DNA fragment. Results: The local agar genusGracilaria gigas,Gelidium, brand B and brand S could separateDNA fragments at a concentration between 1% and 2%, with an optimum concentration of 2% w/v, as good as a specific brand of imported commercial agarose. Conclusions:Their performance were very close to that of commercial agarose and can still be improved by further agar purification as well as by pH and sulfur control.