RESUMO
ABSTRACT Objective: Recent studies have shown a relationship between adipose tissue and coronary artery disease (CAD). The ABCA1 transporter regulates cellular cholesterol content and reverses cholesterol transport. The aim of this study was to determine the relationship between single nucleotide polymorphisms (SNPs) R230C, C-17G, and C-69T and their expression in epicardial and mediastinal adipose tissue in Mexican patients with CAD. Subjects and methods: The study included 71 patients with CAD and a control group consisting of 64 patients who underwent heart valve replacement. SNPs were determined using TaqMan probes. mRNA was extracted using TriPure Isolation from epicardial and mediastinal adipose tissue. Quantification and expression analyses were done using RT-qPCR. Results: R230C showed a higher frequency of the GG genotype in the CAD group (70.4%) than the control group (57.8%) [OR 0.34, 95% CI (0.14-0.82) p = 0.014]. Similarly, C-17G (rs2740483) showed a statistically significant difference in the CC genotype in the CAD group (63.3%) in comparison to the controls (28.1%) [OR 4.42, 95% CI (2.13-9.16), p = 0.001]. mRNA expression in SNP R230C showed statistically significant overexpression in the AA genotype compared to the GG genotype in CAD patients [11.01 (4.31-15.24) vs. 3.86 (2.47-12.50), p = 0.015]. Conclusion: The results suggest that the GG genotype of R230C and CC genotype of C-17G are strongly associated with the development of CAD in Mexican patients. In addition, under-expression of mRNA in the GG genotype in R230C is associated with patients undergoing revascularization.
RESUMO
Para favorecer una alimentación correcta la orientación alimentaria debe considerar factores socioculturales que condicionen patrones y preferencias del consumo de alimentos propios de cada población. El objetivo de esta investigación fue describir el contexto sociocultural del embarazo y su alimentación en el ámbito doméstico, mediante un enfoque cualitativo. Se realizaron entrevistas en profundidad a 6 mujeres con embarazo de alto riesgo bajo la técnica de saturación de la información. Las entrevistas se transcribieron y analizaron de acuerdo a Bardín con ayuda del programa Atlas TI. Se identificó: incertidumbre por el embarazo no planeado y poco apoyo de la pareja; ciertos cambios en la alimentación para mejorar la salud; percepción de una mala y buena alimentación, aunque ésta no cumplía con las características de una dieta correcta; omisión de la cena y del grupo de verduras y frutas. Las encargadas de preparar y servir los alimentos no siempre fueron las mujeres embarazadas; los alimentos percibidos peligrosos fueron consumidos y los benéficos presentaron consumo escaso. Resaltó la necesidad de capacitar a los profesionales de la salud para considerar al contexto sociocultural en la orientación alimentaria que proporcionen.
To promote a healthy diet, the dietary guidance should consider socio-cultural factors as they influence both the food standards and preferences of each population. The aim of this study was to describe the socio-cultural contexts of pregnancy and feeding in the domestic arena, using a qualitative approach. In-depth interviews were conducted using the data saturation technique with six women experiencing high-risk pregnancy. The interviews were transcribed and analyzed in accordance with the Bardin method and the IT Atlas program. The following aspects were identified: uncertainty due to unplanned pregnancy and limited partner support; a few dietary changes to improve health; perception of bad and good feeding habits, although it did not comply with the characteristics of a good diet; omission of fruit and vegetable group from dinner. The people in charge of preparing and serving food were not always the pregnant woman; food products perceived as being harmful were consumed and beneficial products were rarely consumed. This highlighted the need to train professionals to consider the socio-cultural context when providing dietary guidance.