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1.
Zahedan Journal of Research in Medical Sciences. 2015; 17 (2): 52-56
em Inglês | IMEMR | ID: emr-169433

RESUMO

Rhodotorula is characterized by the absence of ballistoconidia, fermentation ability, and starch-like compounds. Biology of the species is not well-identified; Therefore molecular identification is required. Sequence analysis of the D1/D2 region can be used for the identification of the majority of Basidiomycetous species. Carotenoids which are natural pigments can be synthesized by some genera of yeasts such as Rhodotorula. The increase of demand for carotenoids obtained from natural sources has promoted major efforts to recognize potential microbial sources. The aims of this study were to identify a strain isolated from leather wastewater and to investigate its carotenoid production ability. The effect of 2 different medium [Semi-synthetic medium [MMS] and Yeast Malt extract medium [YM]] on biomass and carotenoid production was studied. In this experimental study, Sequence analysis of the D1/D2 region in addition to morphological and biochemical characterization to identify the strain was carried out. To isolate the carotenoid pigment, cells were suspended in acetone and broken using a homogenizer, followed by centrifugation and supernatant was separated; Thus pigments were measured spectrophotometrically at 450 nm using the extinction coefficient E[1%][450]=2500. Identification processes represented strain SG006 as a Rhodotorula slooffiae. The sequence was deposited in the Gene Bank database with accession number JX997835. The results showed that SG006 are able to produce carotenoid and MMS medium promoted carotenoid production. We found that Rhodotorula slooffiae showed the ability to produce carotenoid. However, further work is needed to optimize of the amount of product and to characterize the carotenoids

2.
Razi Journal of Medical Sciences. 2012; 19 (96): 45-56
em Persa | IMEMR | ID: emr-151701

RESUMO

To present an overview of the pharmacologic treatment of pseudophakic macular edema. A systematic review of the currently accepted pharmacologic treatments for pseudophakic macular edema. Main outcome parameters include visual acuity and central macular thickness as determined by ocular coherence tomography. Articles retrieved by Pubmed search using keywords pseudophakic cystoids macular edema, cataract surgery, treatment, vascular endothelial growth factor, Nonsteroidal Anti-inflammatory Drugs, Optical Coherence Tomography, and vitrectomy. New drugs including new nonsteroidal anti-inflammatory drugs [NSAID] and anti-vascular endothelial growth factor [VEGF] agents have been used in the treatment of pseudophakic cystoid macular edema. Furthermore, new routes for drug administration and surgical methods have been used in chronic cases, some showing good effects

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