Neuroprotective Effect of Isobenzofuranones on Hydrogen Peroxide-Mediated Redox Imbalance in Primary Cultures of Hippocampal Neurons

Abstract In live organisms, there is a balance between the production of reactive oxygen species (ROS) and their neutralization. The increased level of these species leads to a condition called redox imbalance. The aim of this study was to evaluate the protective action of isobenzofuranones in primary cultures of hippocampal neurons subjected to redox imbalance. To accomplish this, MTT and LIVE/DEAD assays were initially performed. In the cultures pretreated with isobenzofuranones 1 and 2, there was a higher number of live cells when compared to that in the untreated ones. Regarding redox imbalance, there was a significant increase in the intracellular levels of ROS. The cultures pretreated with isobenzofuranones showed a reduction in ROS levels. Lipid peroxidation caused by oxidative damage was significantly reduced in the cultures pretreated with isobenzofuranones 1 and 2. Taken together, these data show the ability of isobenzofuranones 1 and 2 to significantly minimize cytotoxicity, cell death, intracellular levels of ROS and lipid peroxidation induced by redox imbalance. These results suggest that isobenzofuranones 1 and 2 represent a possible alternative therapy for the neurodegenerative disturbances that are triggered by ROS production increases.

Análise das principais complicações durante a terapia hemodialítica em pacientes com insuficiência renal crônica / Analysis of major complications during hemodialysis therapy in patients with chronic renal failure

Este estudo objetivou analisar as principais complicações durante a terapia hemodialítica em pacientes com insuficiência renal crônica. Tratou-se de um estudo descritivo-exploratório, retrospectivo, documental, com abordagem quantitativa, realizado em um hospital do norte de Minas Gerais, Brasil. A amostra deste estudo foi composta por 309 prontuários de pacientes em terapia renal substitutiva durante o período de julho a dezembro de 2011. Foram identificadas como complicações mais frequentes: hipertensão (n=38; 12,7%), hipotensão (n=13; 4,2%), câimbras (n=9; 2,9%) e cefaleia (n=8; 2,5%), com predominância em pacientes do sexo masculino (n=194; 62,7%) na faixa etária de 50 a 74 anos (n=143; 46,2%). Como observado, a ocorrência de complicações apresentadas pelos pacientes renais durante as sessões de hemodiálise é frequente. Portanto, a constante avaliação dessas complicações deve estar inserida nos programas de controle de qualidade do tratamento, de forma a contribuir com a intervenção eficaz da equipe de enfermagem.

This study aimed to analyze the main complications during hemodialysis in patients with chronic renal failure. This is a descriptive, exploratory, retrospective documentary study with a quantitative approach carried out in a hospital in northern Minas Gerais (MG). The study sample consisted of 309 records of patients on renal replacement therapy during the period July-December 2011. The results showed the following diagnosed complications: hypertension (n=38, 12.7%), hypotension (n=13, 4.2%), cramps (n=9, 2.9%) and headache (n=8, 2.5%), with a predominance of male patients (n=194, 62.7%) aged 50-74 years (n=143, 46.2%). As noted, the occurrence of renal complications presented by patients during hemodialysis is frequent. Therefore, the constant evaluation of these complications should be inserted in the quality control of treatment programs to contribute with the effective intervention of the nursing staff.

Este estudio tuvo como objetivo analizar las principales complicaciones durante la hemodiálisis en pacientes con insuficiencia renal crónica. Se trata de un estudio descriptivo, exploratorio, retrospectivo documental con un enfoque cuantitativo llevado a cabo en un hospital en el norte de Minas Gerais (MG). La muestra del estudio consistió en 309 historias clínicas de pacientes en tratamiento sustitutivo renal en el período de julio a diciembre de 2011. Los resultados mostraron como las más frecuentes complicaciones: hipertensión arterial (n=38, 12,7%), hipotensión (n=13, 4,2%), calambres (n=9, 2,9%) y cefalea (n=8, 2,5%), con un predominio de pacientes del sexo masculino (n=194, 62,7%) de edad 50 a 74 años (n=143, 46,2%). Como se ha señalado, la aparición de complicaciones renales presentadas por los pacientes durante la hemodiálisis es frecuente. Por lo tanto, la evaluación constante de estas complicaciones se debe insertar en el control de calidad de los programas de tratamiento de forma a contribuir en la intervención efectiva del equipo de enfermería.