RESUMO
Se tomaron muestras de exudados vaginales a 100 pacientes femeninas con sospechas de candidiasis vaginal que acudieron al Laboratorio de Microbiología del Hospital Clínico Quirúrgico "Freyre de Andrade" de Ciudad de La Habana. Estas muestras se sembraron en agar Sabouraud y agar Sabouraud trifenil tetrazolio (AST). En 45 % de las muestras se obtuvo crecimiento de colonias levaduriformes, donde Candida albicans (51,1 %) y C. glabrata (22,2 %) fueron las especies aisladas con mayor frecuencia. Se logró demostrar que el AST permite diferenciar los morfotipos de las especies de levadura, y se observó que la mayoría de las especies presentó más de un morfotipo, predominando en C. albicans las colonias lisas, de color rosado claro sin halo micelial alrededor. En este no creció C. glabrata. Esta tipificación fenotípica en AST permitirá abordar estudios epidemiológicos en un futuro.
Samples of vaginal smears were taken from 100 patients suspected of vaginal candidiasis that attended the Microbiology Laboratory of "Freyre de Andrade" Clinical and Surgical Hospital, in Havana City. These samples were cultivated in Sabouraud agar and Sabouraud triphenyltetrazolium agar (STA). In 45 % of the samples it was observed growth of the yeast-shaped colonies, where Candida albicans (51.1 %) and C. glabrata (22.2 %) were the most frequently isolated species. It was proved that STA allowed to differentiate the morphotypes of the yeast species and it was observed that most of the species presented more than one morphotype with the predominance of flat light pink colonies without mycelial halo in C. albicans, C. glabrata did not grow in it. This phenotypic typing in STA will permit to approach epidemiological studies in a future.
Assuntos
Feminino , Humanos , Candida/classificação , Vagina/microbiologiaRESUMO
Se realizó un estudio prospectivo en el Servicio de Cirugía Mayor Ambulatoria y de Corta Hospitalización del Hospital Clinicoquirúrgico Docente "Miguel Enríquez", para conocer el número de intervenciones realizadas por este método, afecciones más frecuentes, tipos de anestesia y complicaciones; para ello se revisaron las historias clínicas de 3 254 pacientes intervenidos de enero de 1991 a diciembre de 1995; se agruparon por niveles, y se pudo observar que predominaron las hernias inguinales en el nivel II y la litiasis vesicular en el nivel III; se presentaron tan sólo 69 complicaciones.
A prospective study was conducted at the Service of Ambulatory Major Surgery and of Short Hospitalization of the "Miguel Enríquez" Clinical and Surgical Teaching Hospital to know the number of operations performed by this method, the most frequent affections, the types of anesthesis and complications. To this end, the medical histories of 3 254 patients who were operated on from January, 1991, to December, 1995, were reviewed, and grouped by levels. There was a predominance of inguinal hernias at level II and of vesicular lithiasis at level III. Only 69 complications were observed.
RESUMO
Physical examination of nails was carried out in 210 elderly patients and nail scrapings were obtained from onychomycosis suggested lesions in order to determine their causative agents, incidence and clinical characteristics. Diagnostic was confirmed by the isolation of the agents from 74 patients, mainly from toe-nails (incidence 35.2). Tinea pedis occurred in 25 of the cases and Diabetes mellitus was the most prevalent associated disease and the most frequent clinical characteristics were the thickening, the opacity and the presence of longitudinal strias in the surface of the nails. It was compared the results obtained by microscopic examination and by culture. Trichophyton rubrum was the most common dermatophyte isolated; Candida parapsilosis was dominant among Candida species.