A meta -analysis on the prevalence of secondhand smoke among Chinese mainland female residents / 预防医学

Objective Using the method of meta -analysis to systematic evaluate the prevalence of secondhand smoke among Chinese mainland female residents,and to provide reference for making relevant smoking control regulations and carrying out targeted tobacco control action.Methods Searching Pubmed,CNKI,Wanfangdata,VIP and CBM database to collect the literatures that were included between 2001 and 2015 about the prevalence of secondhand smoke among Chinese mainland female residents.Meta -analysis was tested by software CMA V2.Results Thirty four literatures were selected with a total of 218180 non -smokers and 99738 secondhand smokers.The pooled prevalence of secondhand smoke among Chinese mainland female residents was 46.88% (95% CI:44.10% -49.67%)from 2001 to 2012.Stratified analysis showed that the prevalence of secondhand smoke were 44.69% and 55.50% for eastern and midwest China,and 40.31% and 51.46% for urban and rural area.The prevalence among urban -rural was statistically significant.The prevalence of secondhand smoke among Chinese mainland female residents was 52.97%,50.06% and 44.30% for 2001-2004,2005 -2008 and 2009 -2012.Conclusion The high prevalence of secondhand smoke among Chinese mainland female residents was high.The prevalence of secondhand smoke was higher in rural area than in urban area.

The measurement of transforming growth factor ?1 mRNA in peripheral blood mononuclear cell of patients with chronic hepatic disease by fluorescence quantitative reverse transcription polymerase chain reaction / 中华检验医学杂志

Objective To develop fluorescence quantitative reverse transcription polymerase chain reaction (FQ-RT-PCR) for detection and quantitation of transforming growth factor ?1 (TGF-?1) mRNA in peripheral blood mononuclear cell (PBMC) of patients with chronic hepatic disease.Methods The TGF-?1 recombined plasmid was constructed by conventional molecular biological techniques,and strand-specific cRNA standard was synthesized by T7 RNA polymerase in vitro transcription.The cRNA standard and a TaqMan-MGB probe were used for quantitation of the TGF-?1 mRNA,and the assay was evaluated.Moreover,the plasma TGF-?1 was detected by ELISA.Results Sequence analysis indicated that the recombined plasmid contains the specific 102bp fragment of TGF-?1. The FQ-RT-PCR could detect as low as 6.81 copies of standard cRNA,the linear range was from 6.81 to 6.81?10~9 copies,and the coefficient variation was 1.28%-2.27% and 2.56%-2.61% respectively in intra and inter-assay.The levels of TGF-?1 mRNA in PBMC and plasma TGF-?1 of patients with chronic hepatic disease were significantly higher than that of healthy controls(P