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1.
Journal of Veterinary Science ; : 523-529, 2016.
Artigo em Inglês | WPRIM | ID: wpr-167766

RESUMO

Members of the genus Anaplasma are important emerging tick-borne pathogens in both humans and animals in tropical and subtropical areas. Here, we investigated the presence of Anaplasma spp. in 621 sheep and 710 goats from six provinces of China. Polymerase chain reaction (PCR) and DNA sequencing were conducted to determine the prevalence of Anaplasma (A.) phagocytophilum, A. ovis and A. bovis targeting the 16S ribosomal RNA or the major surface protein 4 gene. PCR revealed Anaplasma in 39.0% (240/621) of sheep and 45.5% (323/710) of goats. The most frequently detected species was A. ovis (88/621, 14.2% for sheep; 129/710, 18.2% for goats), followed by A. bovis (60/621, 9.7% for sheep; 74/710, 10.4% for goats) and A. phagocytophilum (33/621, 5.3% for sheep; 15/710, 2.1% for goats). Additionally, eight sheep and 20 goats were found to be infected with three pathogens simultaneously. DNA sequencing confirmed the presence of these three Anaplasma species in the investigated areas, and phylogenetic analysis indicated that there was geographic segregation to a certain extent, as well as a relationship between the host and cluster of A. ovis. The results of the present study provide valuable data that helps understand the epidemiology of anaplasmosis in ruminants from China.


Assuntos
Animais , Humanos , Anaplasma ovis , Anaplasma phagocytophilum , Anaplasma , Anaplasmose , China , Epidemiologia , Cabras , Reação em Cadeia da Polimerase , Prevalência , RNA Ribossômico 16S , Ruminantes , Análise de Sequência de DNA , Ovinos
2.
Chinese Journal of Zoonoses ; (12): 747-752, 2014.
Artigo em Chinês | WPRIM | ID: wpr-453250

RESUMO

Entamoeba is a zoonotic protozoan that can parasitize in the intestine and other organs in human and animals . There are many species in this genus ,but only Entamoeba histolytica can cause amebic colitis and liver abscess ,and even death .The cysts and trophozoits are difficult to be discriminated for pathogenic and nonpathogenic Entamoeba .And there is al-so difference in the pathogenicity ,infectivity etc .,even in the same species .Therefore ,it is difficult to confirm the Entamoeba species according to traditional method ,thus affecting the use of medicine .In this article ,the research progress of molecular examination and genotyping method about entamoeba are reviewed .

3.
Chinese Medical Equipment Journal ; (6)2004.
Artigo em Chinês | WPRIM | ID: wpr-587304

RESUMO

The quality of technicians is the dominate factor to determine the level of medical engineering section.The key to improving technical skills is to do basic jobs.During the Tenth Five-Year-Plan,we not only did many basic jobs and research works in science but did a lot of revolutions in order to accelerate the development of medical engineering and improve the working quality.Compared with the past,we have made great progress and development in standardized management,technology support,technician education,scientific researches,studies,technology level,the quality of service,etc.

4.
Chinese Medical Equipment Journal ; (6)2004.
Artigo em Chinês | WPRIM | ID: wpr-586922

RESUMO

The paper puts forward the design and application of an imaging analysis system for micro-particles.The structure of the system,design theory and its application are discussed in the paper.

5.
Chinese Medical Equipment Journal ; (6)2003.
Artigo em Chinês | WPRIM | ID: wpr-587779

RESUMO

This paper introduces the method of electron tomography three-dimensional reconstruction,which is widely applied in structural biology field and summarizes its advantages and disadvantages.The aim is to provide a reference for the application of the method.

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