RESUMO
The Lianhua Qingwen (LHQW) capsule is a popular traditional Chinese medicine for the treatment of viral respiratory diseases.In particular,it has been recently prescribed to treat infections caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).However,due to its complex composi-tion,little attention has been directed toward the analysis of chemical constituents present in the LHQW capsule.This study presents a reliable and comprehensive approach to characterizing the chemical constituents present in LHQW by high-performance liquid chromatography-Q Exactive-Orbitrap mass spectrometry (HPLC-Q Exactive-Orbitrap-MS) coupled with gas chromatography-mass spectrometry(GC-MS).An automated library alignment method with a high mass accuracy (within 5 ppm) was used for the rapid identification of compounds.A total of 104 compounds,consisting of alkaloids,flavonoids,phenols,phenolic acids,phenylpropanoids,quinones,terpenoids,and other phytochemicals,were suc-cessfully characterized.In addition,the fragmentation pathways and characteristic fragments of some representative compounds were elucidated.GC-MS analysis was conducted to characterize the volatile compounds present in LHQW.In total,17 compounds were putatively characterized by comparing the acquired data with that from the NIST library.The major constituent was menthol,and all the other compounds were terpenoids.This is the first comprehensive report on the identification of the major chemical constituents present in the LHQW capsule by HPLC-Q Exactive-Orbitrap-MS,coupled with GC-MS,and the results of this study can be used for the quality control and standardization of LHQW capsules.
RESUMO
Objective To investigate the effects of long non-coding RNA (lncRNA) HOTAIR on the biological behavior of the vulvar squamous cell carcinoma A431 cells.Methods The expression level of HOTAIR in vulvar squamous cell carcinoma A431 cells and normal epidermis HaCaT cells were detected by real-time PCR.A431 cells were transfected with HOTAIR siRNA,then the proliferation,apoptosis,migration activities,and invasion activities were measured by CCK-8 assay,flow cytometry assay,transwell chamber migration test,and transwell chamber invasion test,respectively.Results After down-regulating the level of HOTAIR in A431 cells,the cell proliferation,migration,and invasion abilities were decreased (P ＜ 0.05),while the cell apoptosis rate was increased (P ＜ 0.05).Conclusion lncRNA HOTAIR may play an important role in the proliferation,apoptosis,migration,and invasion in vulvar squamous cell carcinoma.