RESUMO
OBJECTIVE:To develop an RP-HPLC method for the content determination of berberine in Fujie lotion.METHODS:The separation was performed on Agilent 1200 technologies XDB-C18(250 mm?4.6 mm,5?m)column with acetonitrile-0.02%monobasic potassium phosphate(45:55)as mobile phase at flow rate of 1.0 mL?min-1.The UV detection wavelength was set at 265nm.RESULTS:The linear range of berberine were 0.029 6～0.236 8?g(r=0.999 1)with an average recovery of 99.21%(RSD=1.10%,n=9).CONCLUSION:Established method is sensitive,simple,rapid,accurate and reproducible for the quality control of Fujie lotion.
RESUMO
OBJECTIVE:To study the drug-releasing characteristics of acyclovir eye gel in vitro.METHODS:The buffer phosphate was taken as the releasing mediator,the content of the aciclovir in the releasing mediator was determined by HPLC,the drug releasing charactersitic of aciclovir eye gel was studied by bag filter method.RESULTS:The releasing curve of the aciclovir eye gel in vitro was in line with the first-order drug releasing equation.CONCLUSION:The aciclovir eye gel is characterized by slow-release to some degree.
RESUMO
OBJECTIVE:To study the osmotic releasing of acyclovir in isolated cornea of rabbits.METHODS:The cu?mulative osmotic drug releasing amount of acyclovir eye-gels and acyclovir eye-drops in the isolated corneal of rabbits were determined by HPLC with glutathion buffer as releasing medium.Meanwhile the curves of drug releasing were drawn.RE?SULTS:The osmotic drug releasing of acyclovir eye-gels conformed to the first-order kinetics equation.The cumulative osmotic drug releasing amount of acyclovir eye-drops was higher than that of eye-gels at0.25h;however,it worked in re?verse thereafter.CONCLUSION:Within a short time after administration(0.25h),aqua oculi drug concentration of acyclovir eye-drops was higher than that of acyclovir eye-gels,while the retention time of eye-gels was longer and cornea serves as drug reservoir to some degree.