RESUMO
<p><b>OBJECTIVE</b>To evaluate the expression of BRAF V600E mutation in 240 Chinese patients with thyroid lesions.</p><p><b>METHODS</b>Two hundred and forty Chinese patients with thyroid lesions, including 129 papillary thyroid carcinomas (PTC), 12 follicular carcinomas, 4 medullary carcinomas, 30 adenomas, 30 nodular goiters, and 35 papillary hyperplasia. DNA was extracted from thyroid biopsy and paraffin embedded thyroid tissues, and the expression of BRAF V600E mutation was detected by polymerase chain reaction and DNA sequencing assays.</p><p><b>RESULTS</b>The presence of BRAF V600E mutation was found in 61 of the total group of 240 cases (25.4%). It was only detected in PTC (47.3%), and not detected in other types of malignant and benign thyroid lesions. There was a statistically significant difference between the expression of BRAF V600E mutation in classic type PTC (49.6%) and in follicular type PTC (12.5%,P < 0.05), but statistical data did not show any correlation between BRAF V600E mutation and clinicopathologic parameters in PTC (P > 0.05).</p><p><b>CONCLUSIONS</b>BRAF V600E mutation has a significant correlation with PTC and the detection of BRAF V600E mutation may be used as an important prognostic marker of PTC. Our new method of DNA extraction from paraffin embedded tissues is efficient and inexpensive.</p>
Assuntos
Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adenocarcinoma Folicular , Genética , Metabolismo , Adenoma , Genética , Metabolismo , Biomarcadores Tumorais , Genética , Carcinoma Papilar , Genética , Metabolismo , Códon , Análise Mutacional de DNA , DNA de Neoplasias , Genética , Bócio Nodular , Genética , Metabolismo , Mutação Puntual , Proteínas Proto-Oncogênicas B-raf , Genética , Metabolismo , Neoplasias da Glândula Tireoide , Genética , MetabolismoRESUMO
Splenosis is ectopic autotransplantation of splenic tissue after splenic trauma or surgery.(1) The most frequent locations are the surface of visceral peritoneum and parietal peritoneum. Liver and retroperitoneum are rarely involved.(1,2) We present here 2 cases of splenosis involving the liver and retroperitoneum with clinical information, imaging findings, and literature review.