Study on in vitro and in vivo Genotoxicity of Emodin- 8-O-β-D-glucoside / 中国药房

ABSTRACT OBJECTIVE：To evaluate the in vitro and in vivo genotoxicity of emodin-8-O-β-D-glucoside（EG），and to compare the difference of in vitro cell test and in vivo test of rats. METHODS：2D and 3D hepatocyte models were established by in vitro two-dimensional（2D）and three-dimensional（3D）cell culture. After modeling，2D and 3D hepatocyte were divided into blank control group（0.5％ DMSO），mitomycin C group（positive control，0.1 μg/mL），EG low-dose，medium-dose and high-dose groups（10，50，200 μg/mL），respectively. The micronucleus ratio and tail DNA％ of HepaRG cells were detected. SD rats were divided into blank control group（0.5％ sodium carboxymethyl cellulose），ethyl methanesulfonate group（positive control，200 mg/kg），EG low-dose，medium-dose and high-dose groups（100，300，1 000 mg/kg），with 6 rats in each group. They were given medicine intragastrically for consecutive 15 d，once a day. 15 days later，the micronucleus formation rate of bone marrow polychromatic erythrocytes and hepatocytes，the tail DNA％ and tail distance of peripheral blood lymphocytes and hepatocytes were measured. RESULTS：In the in vitro 2D HepaRG hepatocyte model，compared with blank control group，the micronucleus formation rate and tail DNA％ of HepaRG cell were increased significantly in mitomycin C group （P＜0.01）. There was no statistical significance in micronucleus formation rate and tail DNA％ of HepaRG cell among EG groups（P＞0.05）. In 3D HepaRG cell model， compared with blank control group， micronucleus formation rate and tail DNA％ of HepaRG cell were increased significantly in mitomycin C group （P＜0.01 or P＜0.001）， while tail DNA％ of HepaRG cell wasincreased significantly in EG high-dose group（P＜0.01）. In the in vivo test，compared with blank control group，the micronucleus formation rate of bone marrow polychromatic erythrocytes and hepatocytes，the tail DNA％ and tail distance of peripheral blood lymphocytes and hepatocytes were all increased significantly in ethyl methanesulfonate group（P＜0.01）. Tail DNA％ of peripheral blood lymphocytes was increased significantly in EG high-dose group （P＜0.01）. There was no statistical significance in the micronucleus formation rate of bone marrow polychromatic erythrocytes and hepatocytes，the tail DNA％ and tail distance of hepatocytes among EG groups（P＞0.05）；with the increase of dose，there was an increasing trend. CONCLUSIONS：The results of this study suggest that in 2D cell model，EG not lead to chromosome breakage and DNA damage，but the long-term administration and repeated administration in vivo of 3D cell model show that EG has a certain risk of DNA damage，so the evaluation results of 3D HepaRG cell model are more similar to those of rats in vivo. KEYWORDS Emodin-8-O-β-D-glucoside；Genotoxicity；Two-dimensional culture；Three-dimensional culture；Rat；Micronucleus test

Case classification in measles surveillance system under the Two-level Logistic Model / 中华流行病学杂志

Objective To study the prevalence rates of Rash and Febrile Illnesses (RFIs) including measles,rubella,scarlet fever,exanthema subitum and the differences among measles and other RFIs to tentatively formulate the logistic regression model through clinical manifestation.Methods All the suspected cases of measles,rubella,scarlet fever,exanthema subitum reported by the county/prefecture lever hospitals at four counties were collected during March 2011 to February 2012.When setting laboratory confirmed measles as dependent variable and existed symptoms as independent variable,a logistic regression model was formulated and optimal operational point (OOP) chosen,according to the ROC curve.Results A total number of 551 cases were collected but the consistency of measles diagnosis between clinical and laboratory was not satisfied,with Kappa value=0.349,same to the diagnosis of rubella.As for the result from the two-lever logistic regression model,symptoms that related to the confirmation of measles would include cough (OR=5.75),conjunctivitis (OR=3.00),Koplik spot (OR =7.52),lymphadenectasis (OR =0.07),rash after fever (OR=0.07).The area under ROC curve was 0.97 and the optimal operational point was 0.249.Conclusion A logistic regression model was formulated using the clinical symptoms which was resulted in better performance on prediction.As the sample size of this survey was small,the expansion on the scale of investigation and laboratory testings were needed before the types and components of measles-related RFIs be clarified.

A seroepidemiologic analysis of hepatitis B in Sichuan province / 中华流行病学杂志

Objective To understand the changes of hepatitis B infection rates,before and after the hepatitis B vaccine Was included into EPI.and to evaluate the effect of immunization which would lead to the development of a more appropriate hepatitis B control strategy.Methods Seroepidemiologic method,with multi-section random sampling method were chosen.14 sites from 8 counties were involved.2-4 ml of the vein blood was drawn from all the individuals engaged in the study including 3806 samples.HBsAg,anti-HBs.anti-Hbe of the samples were tested with ELISA.Results Standardized positive rates of HBsAg and HBsAb were found as 7.05%and 29.77%respectively with the overall infection rate of HBV as 40.30%.The hepatitis B vaccine coverage of the children under 15 years was 70.73%and the positive rates for both HBsAg and anti-HBS were 2.62%and 56.68%.respectively.The coverage of hepatitis B vaccine among children under 3 years was 83.44%and the positive rates of both HBsAg and anti-HBs were 1.47%and 67.69%respectively.hepatitis B vaccine coverage of children under 3 years was 85.77%.with positive rates of HBsAg and anti-HBs as 1.78%and 75.44%respectively.Conclusion Results from our study revealed that since the introduction of hepatitis B vaccination,the prevalence rates of HBsAg and HBV infeetion had an obvious decline,especially in children aged under 15 years of old,suggesting that some changes had occurred in the epidemic characteristics ofhepatitis B in Sichuan.

Combination of recombinant human endostatin and GP in the treatment of advanced non-small cell lung cancer / 中国基层医药

Objective To observe the efficacy and safety of recombinant human endostatin injection (en-dostar) combined with GP(gemcitabine plus cisplatin)regimen in patients with advanced non- small cell lung cancer (NSCLC). Methods Thirty seven histologically confirmed advanced NSCLC patients were enrolled in the group. The patients were administered with endostar 15 mg from day 1 to 14,gemcitabine 1 000 mg/m2 day 1 and 8,cisplatin 80 mg/m2 on days devided into 1 - 3, repeated 21 days. Each patient should complete two cycles. Results 37 patients were valuable for response. One patient achieved complete response(CR), 15 partial response(PR), 14 stable disease (SD) ,and 7 were found to have disease progression(PD). The total response rate was 43.2% ,median TIP was 5.2 months. The main toxicities was leukopenia. There was no treatment-related death in this series. Conclusion En-dostar combined GP regimen was effective and safe in treatment of advanced NSCLC.

Comparison between SPECT and MRI in detecting skull-base invasion in nasopharyngeal carcinoma / 中华放射肿瘤学杂志

Objective To investigate the ability of single photon emission computed tomography (SPECT) and MRI in detecting skull-base invasion in nasopharyngeal carcinoma. Methods Sixty-one patients with nasopharyngeal carcinoma received whole body and skull-base tomography SPECT, and nasopharynx and skull-base MRI before radiotherapy. The results were double-blind compared and evaluated. Results The overall positive rates of skull-base invasion detected by SPECT and MRI were 51% and 46% (P=0.508). In paitents with headache, cranial nerve palsy or both, the rates were 83% and 86% (P=1.000) ,80% and 80% (P=1.000), 88% and 94% (P=1.000), respectively. In patients with T1+T2 and T3+T4lesions,the rates were 22% and 0(P=0.031) ,74% and 82% (P=0.250) ,repectively. In patients with N0+N1and N2+N3lesions,they were 50% and 48% (P=1.000) ,53% and 40% (P=0.500) ,respectively. The conformation rate between SPECT and MRI was 85%. Binary Logistic regression analysis showed that T stage was a risk factor for positive SPECT(χ2=4.23,P=0.040, OR=3.04). Headache tended to be a risk factor for both positive SPECT and positive MRI (χ2=3.13, P=0.077, OR=4.54;χ2=3.64,P=0.056,OR=12.00). Conclusions The detection sensitivity of SPECT in skull-base invasion in nasopharyngeal carcinoma is equivalent to that of MRI. The consistency between SPECT and MRI is good. Moreover, there is a good correlation between SPECT and symptoms, signs and stage. SPECT of skullbase tomography is necessary for patients with severe headache, negative CT and those who can not receive MRI. When SPECT result is positive,skull-base should be considered to be invaded and should be defined as gross tumor volume in radiotherapy planning.

The clinical analysis of chest wall recurrence of breast cancer after mastectomy / 中国癌症杂志

Background and Purpose:The recurrent rate of breast cancer after mastectomy was 5%~20%,high risk factors were included it could achieve 34%~40%,Chest wall recurrence was the most common.This paper explores the reason for chest wall recurrence of breast cancer after mastectomy,hoping to find an efficient way to prevent and reduce chest wall recurrence after mastectomy.Methods:For 39 patients with local recurrence on the chest wall after mastectomy clinical data was reviewed retrospectively.Results:This group of patients was 5.1% of all breast cancer patients in the same period.Most of recurrences(59.0%)occured within two years affer operation.The recurrent rate of T_1～T_4 was 1.6%、1.9%、9.7% and 37.2% respectively.Rate of chest wall recurrence in patients with negative axillary nodes and positive axillary nodes was 1.3%、7.6%,but if the amount of positive axillary nodes≥4,it was 13.4%.Conclusions:In the patients who had more positive axillary nodes,larger primary tumor and no proper adjuvant therapy,recurrence on the chest wall was seen more often.Adjuvant chemotherapy and postoperative radiotherapy are efficient ways to prevent recurrence on the chest wall.

Value of postmastectomy radiotherapy to chest wall in breast cancer / 中华放射肿瘤学杂志

0.05).The 2 -year and 3-year survival rates were 91.7 %,89.2 % and 85.8%,86.1% in all patients for either gr oup. The 2 -year and 3-year survival rates were 84.2%,81.8% and 72.9%,77.1% in p atients with positive axillary lymph nodes for the two groups, with the differen ces insignificant (Logrank test P=0.663, P=0.9 19).There were no differences in the 2-year and 3-year survivals for patients with stage Ⅲ and over receiving ch est wall irradiation or not and patients who received different doses of chest w all irradiation (Logrank test P=0.449, P=0.764 ). Conclusions Locoregional recu rrence is not reduced and survival rate is not improved by chest wall irradiatio n in this study. The prognostic impact of chest wall irradiation and the optimal target of radiotherapy remains to be substantiated by more randomized trials.