Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Adicionar filtros








Intervalo de ano
1.
Journal of Southern Medical University ; (12): 1328-1333, 2016.
Artigo em Chinês | WPRIM | ID: wpr-256600

RESUMO

<p><b>OBJECTIVE</b>To analyze the whole microbial structure in a case of rampant caries to provide evidence for its prevention and treatment.</p><p><b>METHODS</b>Clinical samples including blood, supragingival plaque, plaque in the caries cavity, saliva, and mucosal swabs were collected with the patient's consent. The blood sample was sent for routine immune test, and the others samples were stained using Gram method and cultured for identifying colonies and 16S rRNA sequencing. DNA was extracted from the samples and tested for the main cariogenic bacterium (Streptococcus mutans) with qPCR, and the whole microbial structure was analyzed using DGGE.</p><p><b>RESULTS</b>The patient had a high levels of IgE and segmented neutrophils in his blood. Streptococci with extremely long chains were found in the saliva samples under microscope. Culture of the samples revealed the highest bacterial concentration in the saliva. The relative content of hemolytic bacterium was detected in the samples, the highest in the caries cavity; C. albicans was the highest in the dental plaque. In addition, 33 bacterial colonies were identified by VITEK system and 16S rDNA sequence phylogenetic analysis, and among them streptococci and Leptotrichia wade were enriched in the dental plaque sample, Streptococcus mutans, Fusobacterium nucleatum, and Streptococcus tigurinus in the caries cavity, and Lactobacillus in the saliva. S. mutans was significantly abundant in the mucosal swabs, saliva and plaque samples of the caries cavity as shown by qPCR. Compared to samples collected from a healthy individual and another two patients with rampant caries, the samples from this case showed a decreased bacterial diversity and increased bacterial abundance shown by PCR-DGGE profiling, and multiple Leptotrichia sp. were detected by gel sequencing.</p><p><b>CONCLUSION</b>The outgrowth of such pathogenic microorganisms as S. mutans and Leptotrichia sp., and dysbiosis of oral microbial community might contribute to the pathogenesis of rampant caries in this case.</p>


Assuntos
Humanos , Anormalidades Múltiplas , Cárie Dentária , Microbiologia , Placa Dentária , Microbiologia , Fusobacterium , Imunoglobulina E , Sangue , Lactobacillus , Leptotrichia , Deformidades Congênitas dos Membros , Microbiota , Mucosa Bucal , Microbiologia , Neutrófilos , Biologia Celular , Filogenia , Reação em Cadeia da Polimerase , RNA Ribossômico 16S , Genética , Saliva , Microbiologia , Streptococcus , Anormalidades Dentárias
2.
International Journal of Oral Science ; (4): 206-211, 2013.
Artigo em Inglês | WPRIM | ID: wpr-358164

RESUMO

Streptococcus mutans is a common Gram-positive bacterium and plays a significant role in dental caries. Tobacco and/or nicotine have documented effects on S. mutans growth and colonization. Sortase A is used by many Gram-positive bacteria, including S. mutans, to facilitate the insertion of certain cell surface proteins, containing an LPXTGX motif such as antigen I/II. This study examined the effect of nicotine on the function of sortase A to control the physiology and growth of S. mutans using wild-type S. mutans NG8, and its isogenic sortase-defective and -complemented strains. Briefly, the strains were treated with increasing amounts of nicotine in planktonic growth, biofilm metabolism, and sucrose-induced and saliva-induced antigen I/II-dependent biofilm formation assays. The strains exhibited no significant differences with different concentrations of nicotine in planktonic growth assays. However, they had significantly increased (P≤0.05) biofilm metabolic activity (2- to 3-fold increase) as the concentration of nicotine increased. Furthermore, the sortase-defective strain was more sensitive metabolically to nicotine than the wild-type or sortase-complemented strains. All strains had significantly increased sucrose-induced biofilm formation (2- to 3-fold increase) as a result of increasing concentrations of nicotine. However, the sortase-defective strain was not able to make as much sucrose- and saliva-induced biofilm as the wild-type NG8 did with increasing nicotine concentrations. These results indicated that nicotine increased metabolic activity and sucrose-induced biofilm formation. The saliva-induced biofilm formation assay and qPCR data suggested that antigen I/II was upregulated with nicotine but biofilm was not able to be formed as much as wild-type NG8 without functional sortase A.


Assuntos
Humanos , Motivos de Aminoácidos , Aminoaciltransferases , Genética , Antígenos de Bactérias , Aderência Bacteriana , Proteínas de Bactérias , Genética , Biofilmes , Cisteína Endopeptidases , Genética , Relação Dose-Resposta a Droga , Mutação , Genética , Nicotina , Farmacologia , Peptidoglicano , Genética , Saliva , Fisiologia , Streptococcus mutans , Sacarose , Farmacologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA