Expression level and its clinical significance of human epidermal growth factor receptor 2, estrogen receptor, progesterone receptor, P53 and Ki-67 in breast cancer tissues of elderly patients / 中华老年医学杂志

Objectives To investigate the expression level and its clinical significance of human epidermal growth factor receptor 2 (C-erbB-2),estrogen receptor (ER),progesterone receptor (PR),Ki67 and P53 in breast cancer tissues of elderly patients.Methods The expressions of C-erbB-2,ER,PR,Ki67 and P53 in 103 specimens of breast cancer tissues were assessed by immunohistochemistry(S-P),and their relationships with clinicopathological factors including tumor size,lymph node metastasis status and clinical stages were statistically analyzed.Results The positive rate of C-erbB-2,ER,PR,Ki67 and P53 in 103 cases was 17.5％ (18/103),64.1％ (66/103),54.4 ％ (56/103),70.9 ％ (73/103) and 66.0 ％ (68/103),respectively.The positive expression rates of C-erbB-2 and P53 were correlated with clinical stages and lymph node metastasis status(P＜0.05).The positive expression rate of Ki67 was correlated with clinical stages(P＜0.05),but not correlated with tumor size and lymph node metastasis status(P＞0.05).The positive expression rates of C-erbB-2,P53,ER and PR were irrelevant to tumor size(P＞0.05).Conclusions The expression of C-erbB-2,ER,PR,Ki67 and P53 could be biological markers for estimating the occurrence and progression of elderly breast cancer.Detection of C-erbB-2,ER,PR,Ki67 and P53 may help judge the clinical stages and lymph node metastasis status in elderly patients with breast cancer.

Relationship and significance between C609 T polymorphism of NQO1 gene and breast cancer molecular subtype / 临床与实验病理学杂志

Purpose To investigate the distribution of polymorphisms of quinone oxidoreductase 1 (NQO1) C609T gene in breast cancer patients, and to analyze the relationship with breast cancer molecular subtype. Methods Genotyping of C609T rs1800566 lo-cus of NQO1 gene in peripheral blood of 248 cases of female breast cancer were detected using high-throughput TaqMan MGB real-time fluorescence quantitative PCR technology, while the detection of ER, PR, HER-2 and Ki-67 in cancerous tissues were used with immu-nohistochemical staining and FISH gene amplification. Results Among 248 cases of breast cancer patients, CC genotype accounted for 27. 42% (68/248), CT genotype accounted for 49. 60% (123/248), TT genotype accounted for 22. 98% (57/248), which con-sistent with Hardy-Weinberg equilibrium law genetic (P>0. 05). 5 cases of HER-2 (++) who did not undergo FISH testing were re-moved, all the rest were done with FISH detection. Luminal A type accounted for 15. 2% (37/243), Luminal B type accounted for 51. 4% (125/243), HER-2 overexpression type accounted for 19. 8% (48/243), basal cell type accounted for 13. 6% (33/243). Compared with patients carrying the CC genotype, ER and PR positive rates in breast cancer patients carrying CT and TT genotype was significantly higher (P0. 05). There was no statistically difference on distribution of C609T polymorphism of NQO1 gene among different molecular sub-types of breast cancer (P>0. 05). Conclusions Here is no relationship between C609T polymorphism of NQO1 gene and breast cancer molecular subtype, miss rate of NQO1 ( CT+TT) in basal cell carcinoma is lower, and its gene polymorphism may provide the reasonable explanation to the heterogeneity of breast cancer molecular subtype.

Clinical significance of HIF-1α, Ki67 and VEGF expression in breast cancer / 中华内分泌外科杂志

Objective To examine the expression of hypoxia inducible factor-1 α (hypoxia-inducible factor 1α,HIF-1α),tumor cell proliferation antigen Ki67 and vascular endothelial growth factor (VEGF) in breast cancer tissues,adjacent tissues and normal breast tissues.Methods Immunohistochemistry HRP method was used to detect the expression of HIF-1α,Ki67 and VEGF in 70 cases of breast cancer tissues and their adjacent tissues and 10 cases of normal breast tissues.Difference of the positive rate was assessed viax2 test,and the correlation between the 3 were analyzed in a disorderly classified manner.Results The positive expression rate of HIF-1α,Ki67 and VEGF in 70 cases of breast cancer tissues was 78.6％ (55/70),88.6％ (62/70) and 65.7％ (46/70) respectively,significantly higher than that in adjacent tissues and normal breast tissues (P ＜ 0.01).The positive expression rate of HIF-1 α,Ki67 and VEGF was closely related with clinical stage,lymph node metastasis and histological grade (P ＜0.05).The expression intensity of HIF-1α was positively correlated with Ki67 and VEGF in breast cancer (P ＜ 0.05).Conclusions Detection of HIF-1 α,Ki67,VEGF may be an important indicator to predict the development,invasion,metastasis and prognosis of breast cancer.Tissue hypoxia may promote the proliferation of breast cancer cell and angiogenesis.A presumption is that HIF-1αmay be a new t()get of breast cancer,thus it deserves further study.