RESUMO
Objective To study effect of high titer cold lectin on positive and negative typing of ABO blood group and electron cross matching test .Methods A total of 500 patients needed emergency blood transfusion were selected from November 2015 to October 2016 in People′s Hospital of Qinbei District .To observe the results of electron crossover and serum cross primary and sec-ondary matching ,ABO blood group positive and negative and electronic cross matching experimental results at different tempera-tures ,cold agglutinin test erythrocyte agglutination and cross matching results .Results There was no significant difference on the main side between the electronic cross matching and serological cross matching (P>0 .05) ,but the difference was significant on sec-ondary side(P<0 .05) .In electron cross test ,18 cases appeared agglutination at 20 ℃ ,which was obviously more than that at 37 ℃(P<0 .05) .Patients blood in the positive stereotypes A type ,anti stereotypes B type ,O type all appeared agglutination ,and at 20 ℃O type and self control also appeared agglutination .Cold agglutinin test results showed that when the temperature was lower than 31 ℃ ,red cell agglutination occurred ,when the temperature was lower than 4 ℃ ,visible agglutination ,but when the temperature rose to 37 ℃ ,agglutination disappeared .Conclusion High titer cold lectin could promote the aggregation of red blood cell at low temperature conditions ,and effect the results of ABO blood group positive and negative and electronic cross matching test ,then effect transfusion therapy .
RESUMO
Objective To study effect of high titer cold lectin on positive and negative typing of ABO blood group and electron cross matching test .Methods A total of 500 patients needed emergency blood transfusion were selected from November 2015 to October 2016 in People′s Hospital of Qinbei District .To observe the results of electron crossover and serum cross primary and sec-ondary matching ,ABO blood group positive and negative and electronic cross matching experimental results at different tempera-tures ,cold agglutinin test erythrocyte agglutination and cross matching results .Results There was no significant difference on the main side between the electronic cross matching and serological cross matching (P>0 .05) ,but the difference was significant on sec-ondary side(P<0 .05) .In electron cross test ,18 cases appeared agglutination at 20 ℃ ,which was obviously more than that at 37 ℃(P<0 .05) .Patients blood in the positive stereotypes A type ,anti stereotypes B type ,O type all appeared agglutination ,and at 20 ℃O type and self control also appeared agglutination .Cold agglutinin test results showed that when the temperature was lower than 31 ℃ ,red cell agglutination occurred ,when the temperature was lower than 4 ℃ ,visible agglutination ,but when the temperature rose to 37 ℃ ,agglutination disappeared .Conclusion High titer cold lectin could promote the aggregation of red blood cell at low temperature conditions ,and effect the results of ABO blood group positive and negative and electronic cross matching test ,then effect transfusion therapy .