In-vitro concentration dependent linear effect of crude snake venom on protein disintegration

The effect of different concentrations [10 microg, 25 microg, 50 microg/ml] of crude snake venom on protein contents of different normal tissues of rabbit was studied. Our objective was to determine and compare the effectiveness of snake venom [SV] on normal tissues before its use in vivo for the treatment of different diseases, especially cancers. Surgical portions of normal tissues of liver, heart, and kidney were removed from rabbits and homogenized. All homogenate samples were incubated with and without SV for 30 minutes at 37°C and measured with the spectrophotometer [Spectron 21]. The in vitro effect of cobra snake venom on total proteins was quantitatively evaluated in different normal rabbit tissues. The different concentrations of SV i.e. 10 microg, 25 microg, and 50 microg/ml demonstrated a significant linear increase in protein concentrations against controls. Significant and linear increases in protein concentrations suggest multiple sites/receptors for venom to act and disintegrate the macromolecules of proteins. The increase in the numbers of small proteins/peptides provides evidence for the venom-specific actions. A concentration of 10 micro g/ml could be of therapeutic potential

potential of the crude snake venom in treatment of human breast cancer with and without combination of Anticancer drugs

Clinical usefulness of the snake venom has been worked out since the last many decades to treat blood pressure, cancer of breast, ovary and etc. we wanted to evaluate the effect of natural compounds of cobra snake venom in vitro on nucleic acid in normal and breast cancer tissues in combination with and without anticancer drugs. Surgically removed tissue from cancerous and non cancerous of same human breast were homogenized and extraction of nucleic acids were prepared. All homogenate samples were incubated with and without snake venom and antineoplastic drugs [Cyclophosophamide and Mitomycin-C] for 30 min at 37 °C and measured at 490 m micro for DNA and at 660 m micro for RNA by spectrophotometer [Sspectron-21[R]]. The effect of the snake venom was compared singly as well as in combination with the two chemotherapeutic agents viz mitomycin-C [an antibiotic] and Cyclophosophamide [an alkylating agent] on the nucleic acids [RNA and DNA]. In human breast cancer tissue shows significant reduction in nucleic acids contains when treated with venom [25 micro g/ml] in combination and compared with singly use anticancer drugs. Cyclophosophamide and Mitomycin produced some effect in human breast tissue at 10 micro g/ml on the DNA only for short time and eventually destroyed. However snake venom does have potential and is more effective with or without anticancer drugs on both nucleic acids [RNA and DNA] suggests wide range of receptors to act. Both drugs could not produce inhibition or potentiating effect on normal and cancerous tissues in vitro by all different doses. Anticancer drugs [Cyclophosophamide and Mitomycin-C], specific medicines for solid tumour [Ca. Breast] used in this study showed no significant reduction at the same dose of snake venom [25 micro g/ml] in vitro in comparison to snake venom. However drugs at 10 micro g/ml has shown some effect on the DNA but only for short time and possibly, eventually are destroyed. However significant and promising anticancer effect of snake venom seems to have a better future as an alternative. None toxic dose of snake venom affects on both DNA and RNA

Invitro crude cobra snake venom significantly decreases the production of RNA and DNA in breast cancerous tissue

Crude cobra snake venom at the rate of 25 micro g / ml reduces nucleic acids production in human breast cancerous tissue invitro. It suggests an ideal model for examining the anticancer activity and could be a better substitute in comparison to presently available anti tumour drugs, for therapeutic use in breast cancer in future

Effect of snake venom on nucleic acids and total proteins in various normal and cancerous animal tissues

Venoms have occasional medical uses. This study was carried out to determine effect of cobra snake venom on nucleic acid and total proteins in various normal and cancerous animal tissues. In this in vitro study the venom with varying concentrations was incubated with homogenates of liver, heart and kidney of normal tissues. Reduction in RNA / DNA levels was observed. Our results indicate that nucleic acids are more sensitive. However the effect of snake venom on normal skin tissue was insignificant when compared with cancerous tissue. The dose response curve shows that the lowest concentration of venom at 25 micro g / ml [a survival dose] produced maximum inhibition of both nucleic acids. The opposite and linear response was observed in protein contents. It can be assumed from the present study that the venom might have therapeutic effect at a dose of 25 micro g /ml in cancerous tissues

Suppression of cell proliferation by snake venom could be related with a fall of nucleic acid content in DMBA - induced rabbit skin carcinoma

Venom has been reported to suppress the cell proliferation, a process which leads to the genesis of cancer. An attempt has been made to observe the in vitro effect of different concentrations [10, 25, 50 microg/ml] of crude cobra snake venom [ccsv], oil contents of RNA and DNA of normal and cancerous [DMBA- induced skin cancer] tissues. The venom failed to demonstrate any change in nucleic acid content of the normal [control] rabbit skin as against DMBA-affected skin. A significant fall in nucleic acid concentration, more pronounced with 25 microg/ml dose, was evident. The data thus suggest that crude snake venom do have a direct suppressant effect on nuclear processes which lead to cell proliferation

Comparative effeicacy and potency of snake venom, mitomycin-C and cyclophosphamide for RNA and DNA inhibition in breast cancer

Anti-proliferating effect of the cobra venom throughh the meassurement of tissue nucleic acids [RNA and DNA] in breast cancerr has been studied and compared to that of cyclophosphamide and mitomycin-C. The venom and other anti-neoplastic were incubated singly as well as in combination for 30 minutes at 37