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By Analyzing the significance and objective,practicing the organizing and management of the clinical application teaching in department of gynecology,the author explored the new teaching methods in current social condition.
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【Objective】To observe the therapeutic effect of laparoscopic ovarian electrocauterization(LOE) combined with cycle treatment of traditional Chinese medicine(TCM-CT) for type phlegm-damp refractory polycystic ovary syndrome(PCOS).【Methods】Forty PCOS patients admitted from August,2004 to March,2006 were randomized into groups A,B and C.Group A(N=20) received LOE and TCM-CT,group B(N=10) received LOE and western medicine for ovulation,and group C received LOE only.The endocrine hormones levels,ovulation rate and pregnancy rate were observed.【Results】The differences of serum luteinizing hormone(LH) and testosterone(T) levels before and after treatment were significant in group A(P0.05).After treatment,LH and T levels in group A differed from those in groups B and C(P0.05).The pregnancy rate in group A differed from those in groups B and C(P0.05).【Conclusion】LOE combined with cycle treatment of traditional Chinese medicine exerts a certain effect on improving reproductive health of patients with type phlegm-damp refractory polycystic ovary syndrome.
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Objective To investigate the effect of ligustrazine on the intracellular translocation of Smad protein in HSC-T6 cell line.Methods HSC-T6 cell was cultured with ligustrazine at the dose of 10-5 mol/L in the culturing dish for 2 hours.After the culturing,the translocation of Smad-2 and Smad-4 proteins was detected by immunofluorescence assay.Results There was no evidence of the translocation of Smad-2 and Smad-4 proteins in HSC-T6 cell after the culturing.Conclusion Ligustrazine can block the signal pathway of TGF-?/Smad,which may be one of its important mechanisms of inhibiting the proliferation of HSC-TS cell.