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Objective:To comprehensively collect the clinical research evidence of Wuqinxi, and provide reference for the future clinical research.Methods:China National Knowledge Infrastructure (CNKI), WanFang Database, VIP, China Biology Medicine disc (CBM), PubMed, Embase, Cochrane Library, and Web of Science were searched. The retrieval time limit was from the establishment of each database to March 31, 2021. After screening and extracting, SPSS 22.0 was used to analyze the basic characteristics, sample size, control and intervention measures, outcomes.Results:Totally 230 studies were included, of which 58.26% (134/230) were randomized controlled trial. The top 6 diseases were chronic obstructive pulmonary disease, osteoporosis, cervical pain, low back pain, diabetes and knee osteoarthritis. Common outcome included four items of blood lipid tests, blood glucose, immunological indicators and the evaluation of disease-related symptoms, such as pain, sleep and range of motion. The median frequency of Wuqinxi was once a day, 45 minutes each time, 5 days a week and lasted for 3 months. 94.78% (218/230) of the studies reported positive results. The safety and compliance of Wuqinxi intervention were good.Conclusions:The number of clinical studies on Wuqinxi is significantly less than Baduanjin and Taijiquan. It is necessary to formulate the report specification of intervention measures for Wuqinxi, so as to report the intervention plan of Wuqinxi more clearly and transparently.
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Purpose The aim is to develop a new method for the determination of the activity of hepatocyte growth factor (HGF). Met hods Rat hepatocytes were prep ared by collagenase perfusion and incubated at 5% CO2 incubator for 1.5 h. The n HGF was added into the culture medium and incubated at 5% CO2 incubator for 1.5 h. The cell viability was determined by MTT method.Results ～1 142 μg/ml improved the number of cell viability significantly （AHGF/Acontrol） ＞2.Conclusion Th is new method was stable, time-saving and of good reappearance. It was adapted to the determination of HGF activity.
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OBJECTIVE:To determine the contents of chloromycetin and dexamethasone acetate in Jiefu cream.METHODS:A reversed-phase HPLC was established for the determination with C18 column as stationary phase.The mobile phase consisted of acetonitrile and 0.05%mol/L phosphate buffer solution(40∶60),the detection wavelength was 240nm.RESULTS:Chlorom-ycetin had a good linearity (r=0.9 998) in the range of 0.1～0.3mg/ml,the mean recovery was 100.6% with a RSD of 1.3%.Dexamethasone acetate had a good linearity(r=0.9 998) in the range of 0.02～0.08mg/ml,the mean recovery was 101.1% with a RSD of 1.7%.CONCLUSION:The method is simple,sensitive and accurate.