RESUMO
BACKGROUND:Stem cell factor(SCF)serves as a factor having a promotion effect on proliferation and differentiation of stemcells.Whether SCF affects biological features of dental pulp stem cells(DPSCs),and plays an important role in tooth regeneration remain unclear.OBJECTIVE:To observe SCF effects on the proliferation and osteogenic difierentiation of human DPSCs.METHODS:Pulp tissues were removed from healthy human teeth extracted for orthodontic purposes.The pulp tissues were digested by collagenase and dispase.The sample was cultivated in culture flasks with medium contained 1,1 0 μmol/L SCF;the normal culture medium served as control.The cell proliferation ability was detected by MTT.The osteogenic gene bone sialoprotein and osteocalcin mRNA expression were examined by real-time PCR.Alkaline phosphatase a ctivity was detectedr by alkaline phosphatase kit.RESULTS AND CONCLUSION:SCF could enhance the proliferation ability,upregulate bone sialoprotein and osteocalcin mRNA expression,and enhance the alkaline phosphatase activity,with the tendency of increased promotion with Increased concentration.Resuits indicated that SCF could enhance the proliferation and osteogenic difierentiation ability of human DPSCs.
RESUMO
<p><b>OBJECTIVE</b>The purpose of this study was to offer pathological reasons for partial parotidectomy adopted in parotid pleomorphic adenoma treatment.</p><p><b>METHODS</b>Continuous slices from 25 cases of primary parotid pleomorphic adenoma were carefully examined and the extra-envelope infiltration, the budding and the primary multi-center were measured and recorded.</p><p><b>RESULTS</b>The range of extra-envelope infiltration and budding was limited in 0.09-0.285 mm out of tumor capsules, which was far less than the safe boundary(1 cm) of the partial parotidectomy. Two cases with multi-centers were found, but all the centers were aggregated together and encapsulated in a united envelope.</p><p><b>CONCLUSION</b>Partial parotid parotidectomy is a safe method and can remove the tumor completely in parotid plemorphic adenoma treatment.</p>