Effect of depot medroxyprogesterone [DMPA] on body weight and serum lipid profile in adult female rats

Depo Provera [Medroxyprogesterone Acetate, DMPA] is a long-acting, injectable progesterone derivative contraceptive that is currently used by 30 million women in 90 countries, including Egypt, United States and Canada. DMPA classified as sex hormone binding globulin [SHBG]. The objective of this study was to investigate body weight and serum lipid profile following treatment of rats with the injectable contraceptive depot medroxyprogesterone acetate, [Depo Provera; DMPA]. Adult female rats [Rattus norvigcus] were treated with a single dose of DMPA that is comparable to the weight-corrected dose in humans [2.7 mg/rat or 5.4 mg/rat] and sacrificed on day ten or fifteen post treatment. Body weights were recorded and sera from both treated and control groups studied for lipid profile using a colorometric method. Treating animals with 2.7 mg/rat or 5.4 mg/rat of DMPA for 10 and 15 days led to a dose-related increase in the body weights [maximum 24.77%]. There was increase in total lipids [TL], triglycerides [TG], total cholesterol [TC] as well as low density-lipoprotein cholesterol [LDL-C] and very low density lipoprotein [VLDL-C]. On the other hand, a marked decrease was found in high density-lipoprotein cholesterol [HDL-C] in the DMPA-treated groups. Statistical analysis indicated that these changes were significant [p < 0.01] in all treated groups compared to control ones. We concluded that, this preliminary study indicates a long-term atherogenic role for DMPA

comparative study on the effect of emdogain and calcium hydroxide on apical closure of young permanent teeth of dogs

The aim of this study was to compare the influence of Emdogain versus Calcium hydroxide on apexification and periapical healing of teeth in dogs with incomplete root formation and previously contaminated canals. twenty premolar of two 6 month old dogs were included. After access to the root ca nals and complete removal of the pulp, the canal system remained exposed to the oral environment for 2 weeks, then all infected canals were cleaned chemically and mechanically and shaped. The.treated teeth were divided into two experimental groups of 20 canals each. In group I, canals were filled with Emdogain. In group II, canals were filled with calcium hydroxide. The animals were sacrificed 3 months post operatively. The blocks of teeth and surrounding tissues were studied to evaluate five parameters: formation of an apical calcified tissue barrier, inflammatory reaction, extrusion of root canal filling, root and bone resorption. The total amount of reparative dentin formed in both groups was calculated using histometric analysis. significant differences [P < 0.05] were found in relation to formation of apical calcified tissue barrier, thickness of calcified tissue barrier, inflammatory reaction and material extrusion. The barrier was formed in 100% of canals from Emdogain group and 75% of canals from calcium hydroxide group. Mild inflammatory reaction and extrusion occurred mainly in roots from group 2. The total amount of reparative dentine formed in Emdogain treated teeth was significantly higher [P < 0.05] than Calcium hydroxide treated specimens. Emdogain showed promising results as a valuable material for apexification, primarily for treating immature teeth with necrotic pulps. It favored the formation of an apical calcified tissue barrier and periapical healing