RESUMO
Background: Antimicrobial peptides with relative length [2-100 amino acids] and positive charge [pure charge [+9]-[+2]] are amphiphilic that isolated from a wide range of animals. Recently, these peptides have been known as a part of innate immune response. Nowadays, more than 500 antimicrobial peptides from animals have been reported
Objectives: The aim of this study was to evaluate the anti-Candida effects of cationic peptides derived from Rana ridibunda skin
Methods: In this study, using alcohol-acid technique, peptides of frog's skin were isolated and purified by Sep-Pack and Sephadex column. Then the anti-Candida activity [Fluconazole Resistance C. albicans, Fluconazole Sensitive C. albicans, and C. glabrata] of the peptides in different concentrations were evaluated
Results: Regarding to statistical analysis, peptides in concentration ranging from 25 to 100 micro g/ml had the most anti-Candida activities. In respect to different understudy Candida species, these agents had the less effect on the Fluconazole Resistance C. albicans [p<0.05]
Conclusions: The anti-Candida effects of cationic peptide obtained from frog skin are approved in this study
RESUMO
The present study investigated the possibility of replacing salamon with modified shotor diluent [MSD] and egg yolk [EY] with low density lipoprotein [LDL] for chilled storage of ram semen. Good quality semen [>80% progressive forward motility [PFM] of sperm] from 3 fertile rams was collected using an artificial vaginaandpooled for each experiment. Low density lipoprotein was extracted from fresh EY. In experiment 1, semen was divided into 2 fractions and extended in MSD or salamon. In experiment 2, semen was assigned into 5 fractions and extended in MSD supplemented with 12 and 15% EY or 3, 5 and 8% LDL. In experiment 3, semen was divided into 2 fractions and extended in MSD supplemented with 12% EY or 5% LDL. Viability of sperm was assessed at times 0 [immediately after semen dilution], 2 or 4 [at 4°C] and up to 72 h after semen dilution. Data was analyzed using General Linear Model [GLM] procedure, including repeated measures. In experiment 1, the viability of sperm was similar in two diluents [P>0.05]. In experiment 2, PFM of sperm was similar among groups at the time of dilution [P>0.05]; but remained elevated in 5 and 8% LDL compared to other groups afterward [P<0.05]. In experiment 3, PFM of sperm was superior at 48 and 72 h after dilution in 5% LDL compared to 12% EY [P<0.05]. In conclusion, MSD supplemented with 5% LDL is a suitable diluent for ram fresh semen preserved at 4°C for 72 h. Key words: Ram, Semen preservation, Low density lipoprotein, Diluent