[Implantation of systemically infused mesenchymal stem cells in rat's bone]

Background: This study proposed to examine the possibility of homing of bone marrow derived-mesenchymal stem cells after allograft transplantation in irradiated and healthy bone marrows

Materials and Methods: 65 rats were divided into 13 groups [1 healthy and 12 irradiated]. Mesenchymal stem cells were isolated from femoral bone marrow of male rats. The cells were cultured under morphological observations, differentiation tests and immunocytochemistry staining. Then, the mesenchymal cells were injected to the tail vein of the healthy and irradiated [7Gy gamma ray] female rats. During defined time gaps after injection, the transplanted rats were killed and their isolated bone marrow was cultured. DNA of the cultured cells was extracted and was amplified by PCR with specific primers for chromosome Y [SRY]. Finally, the PCR products were analyzed on gel electrophoresis

Results: Morphological observations and, differentiation and immunocytochemistry tests conformd that the isolated cells were mesenchymal stem cells. PCR results for the healthy and irradiated rats in all time gaps after transplantation were negative indicating that male mesenchymal stem cells were failed to home in bone marrow of transplanted female rats

Conclusion: We concluded that due to low number of the injected mesenchymal stem cells or trapping of the cells in other organs Implantation the cells in bone marrow was not considerably detectable

Comparison of the efficacy of oral and injectable forms of prophylactic antibiotics in grade II traumatic ulcers in emergency wards of university hospitals of Yazd

Traumatic ulcers are one of the most common causes of referral to emergency wards and interfere with wound healing. Even in a complete sterile condition, all of the ulcers may be contaminated with bacteria, but a few of them progress and cause clinical manifestations. There is a controversy on the use prophylactic antibiotics in traumatic ulcers. In this study we compare the efficacy of oral and injectable forms of antibiotics in prophylaxis of infection. In this clinical trial study, 237 cases suffering from grade II traumatic ulcers were selected by simple random method and divided into 2 groups; first group was administered 1 gram cephazoline prior to suturing and received no other antibiotics, while the second group received 500 mg cephalexin capsule before suturing and continued the treatment for 24 hours. [500 mg QID] .Patients were followed up on day 7, 10 and 30 after discharge from hospital for infection of the wounds. The collected data was analyzed by SPSS 11 software using Chi-squire and Fisher exact tests. According to the findings, confounding variables such as sex, age, width of the wound, traumatic cause and site and also the time course until referral to the emergency ward were similar in both groups. Prevalence of infection in the group receiving oral and injection forms of antibiotic was 2.5% and 1.7%, respectively, difference of which was not significant.[P = 0.683]. As the prevalence of wound infection is similar in both groups, oral forms of antibiotics can be used instead of injectable forms for wound infection prophylaxis

[Evaluation of prehospital care in management of traumatic patients referred to Shahid Rahnemoun and Afshar hospitals of Yazd]

Trauma is the most common cause of mortality in the 1 to 44 years age group and third leading cause of mortality in the world. In general, determination of defects caused by trauma has an essential role in management of traumatic patients. Use of scoring systems for evaluation of static and dynamic states of traumatic events can decrease the mortality and morbidity rates. Different methods are used to decrease the mortality and morbidity rates of trauma in the world. The purpose of this study was to asses the prehospital care of various helper groups, [acquaintances, strangers and 115 service men] and its outcome in traumatic patients. The study was descriptive-analytic, cross-sectional one that was done from the Iranian calendar year 1379 to 1380. 120 patients with multiple traumas were entered in the study. Sampling method in this study was census. The first step was completion of the first page of the questionnaire by interviewing the patient and the accompanying person. After that, according to injury severity score, all defects of the patients were evaluated. RTS [Revised Trauma Score] and TRISS [Trauma Injury Severity Score] were calculated. After filling the questionnaires, data was collected and analyzed by SPSS software. 120 patients with multiple traumas were entered in the study. [107 male and 13 female]. Mean age was 29.6 years, 85.8% had blunt trauma, while 14.2% had penetrating trauma. The average ISS was 10.38, RTS was 7.53, TRSS-B was 93.64 and TRSS-P was92.56. 12 patients required intubations which was done correctly in 5 patients, but was not good in 7 of them. Also, 55 patients required serum therapy that was done appropriately in 45 patients. From all of the 78 patients who needed bleeding control, it was done well for 62 of them. The maximum RTS in patients who died was 6.90. In most situations, helper groups are not trained properly resulting in higher mortality rate. Even though the number of cases was small in the study, it is felt that continual education is needed for on call personnel so that patients in bad condition transported by them are given better care and attention